高滴度嗜双性逆转录病毒生产细胞克隆对人造血祖细胞的转导效率低下。

Poor transduction efficiency of human hematopoietic progenitor cells by a high-titer amphotropic retrovirus producer cell clone.

作者信息

Xu L C, Young H A, Blanco M, Kessler S, Roberts A B, Karlsson S

机构信息

Molecular and Medical Genetics Section, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.

出版信息

J Virol. 1994 Nov;68(11):7634-6. doi: 10.1128/JVI.68.11.7634-7636.1994.

DOI:10.1128/JVI.68.11.7634-7636.1994

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237215/

Abstract

The transduction efficiency of human bone marrow CD34+ cells with supernatants from the retrovirus producer cell clone PA317/LGSN 16 was only one-fifth of that with supernatants from GP+ envAm12/LGSN 15, even though both producers had similar infection titers on 3T3 cells. PA317/LGSN 16-conditioned medium inhibited the proliferation of the bone marrow CD34+ cells, and this inhibitory effect was partially blocked by anti-transforming growth factor beta antibodies. These studies suggest that cytokine secretion plays a role in the suppression of retrovirus transduction of human CD34+ cells.

摘要

尽管逆转录病毒产生细胞克隆PA317/LGSN 16和GP+ envAm12/LGSN 15的上清液对3T3细胞的感染滴度相似，但用PA317/LGSN 16的上清液转导人骨髓CD34+细胞的效率仅为用GP+ envAm12/LGSN 15的上清液的五分之一。PA317/LGSN 16条件培养基抑制骨髓CD34+细胞的增殖，抗转化生长因子β抗体可部分阻断这种抑制作用。这些研究表明，细胞因子分泌在抑制人CD34+细胞的逆转录病毒转导中起作用。

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本文引用的文献

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Correction of the enzyme deficiency in hematopoietic cells of Gaucher patients using a clinically acceptable retroviral supernatant transduction protocol.采用临床可接受的逆转录病毒上清转导方案纠正戈谢病患者造血细胞中的酶缺陷。

Exp Hematol. 1994 Feb;22(2):223-30.

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A safe packaging line for gene transfer: separating viral genes on two different plasmids.一种用于基因转移的安全包装生产线：将病毒基因分离到两个不同的质粒上。

J Virol. 1988 Apr;62(4):1120-4. doi: 10.1128/JVI.62.4.1120-1124.1988.

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Construction and use of a safe and efficient amphotropic packaging cell line.安全高效的嗜性逆转录病毒包装细胞系的构建与应用

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Infection and replication of HIV-1 in purified progenitor cells of normal human bone marrow.

Science. 1988 Nov 11;242(4880):919-22. doi: 10.1126/science.2460922.

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