O'Neill J P, Nicklas J A, Hunter T C, Batson O B, Allegretta M, Falta M T, Branda R F, Albertini R J
Vermont Cancer Center, University of Vermont, Burlington 05401.
Mutat Res. 1994 Oct-Dec;313(2-3):215-25. doi: 10.1016/0165-1161(94)90052-3.
The frequency of 6-thioguanine resistant (TGr) mutant T-lymphocytes arising in vivo in humans can be quantified with a cell cloning assay. However, the in vivo proliferation of T-lymphocytes that may include TGr mutant cells can distort the relationship between mutation events and the resulting frequency of mutant cells. The T-cell receptor (TCR) gene rearrangement pattern of T-cell colonies can be used as an independent measure of clonality. Analysis of T-cell 'clonality' in 413 wild type and 1736 TGr mutant isolates from 58 individuals shows that mutant clonality is a frequent occurrence (35/58 individuals = 60.3%). However, a major effect on the mutant frequency corrected for clonality (the calculated 'mutation frequency') was found only in nine samples all of which had mutant frequencies greater than 40 x 10(-6).
人体内6-硫鸟嘌呤抗性（TGr）突变T淋巴细胞在体内出现的频率可用细胞克隆试验进行定量。然而，可能包括TGr突变细胞的T淋巴细胞在体内的增殖会扭曲突变事件与突变细胞最终频率之间的关系。T细胞集落的T细胞受体（TCR）基因重排模式可作为克隆性的独立指标。对来自58名个体的413个野生型和1736个TGr突变分离株的T细胞“克隆性”分析表明，突变克隆性很常见（35/58名个体 = 60.3%）。然而，仅在9个样本中发现了对经克隆性校正的突变频率（计算得出的“突变频率”）有重大影响，所有这些样本的突变频率均大于40×10⁻⁶。
