一种用于确定与RNA杂交的核酸类似物结构的方法。2'-O-甲基RNA与含有单个酰胺主链修饰的寡核苷酸之间双链体的核磁共振研究。
An approach to the structure determination of nucleic acid analogues hybridized to RNA. NMR studies of a duplex between 2'-OMe RNA and an oligonucleotide containing a single amide backbone modification.
作者信息
Blommers M J, Pieles U, De Mesmaeker A
机构信息
Ciba-Geigy AG, Basel, Switzerland.
出版信息
Nucleic Acids Res. 1994 Oct 11;22(20):4187-94. doi: 10.1093/nar/22.20.4187.
Abstract
The backbone modification amide-3, in which -CH2-NH-CO-CH2- replaces -C5'H2-O5'-PO2-O3'-, is studied in the duplex d(G1-C2-G3-T4.T5-G6-C7-G8)*mr(C9-G10-C11-A12-A13-C14-G15+ ++-C16) where . indicates the backbone modification and mr indicates the 2'-OMe RNA strand. The majority of the exchangeable and non-exchangeable resonances have been assigned. The assignment procedure differs from standard methods. The methyl substituent of the 2'-OMe position of the RNA strand can be used as a tool in the interpretation. The duplex structure is a right-handed double helix. The sugar conformations of the 2'-OMe RNA strand are predominantly N-type and the 2'-OMe is positioned at the surface of the minor groove. In the complementary strand, only the sugar of residue T4 is found exclusively in N-type conformation. The incorporation of the amide modification does not effect very strongly the duplex structure. All bases are involved in Watson-Crick base pairs.
摘要
对主链修饰酰胺-3进行了研究，其中-CH2-NH-CO-CH2-取代了-C5'H2-O5'-PO2-O3'-，研究对象为双链d(G1-C2-G3-T4.T5-G6-C7-G8)*mr(C9-G10-C11-A12-A13-C14-G15+ ++-C16)，其中.表示主链修饰，mr表示2'-OMe RNA链。大多数可交换和不可交换的共振峰已被归属。归属过程与标准方法不同。RNA链2'-OMe位置的甲基取代基可作为解释中的一种工具。双链结构为右手双螺旋。2'-OMe RNA链的糖构象主要为N型，且2'-OMe位于小沟表面。在互补链中，仅残基T4的糖完全以N型构象存在。酰胺修饰的引入对双链结构的影响不是很强。所有碱基都参与了沃森-克里克碱基对。
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