Seiwert S D, Stuart K
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06536-0182.
Science. 1994 Oct 7;266(5182):114-7. doi: 10.1126/science.7524149.
RNA editing in the mitochondrion of Trypanosoma brucei extensively alters the adenosine triphosphate synthase (ATPase) subunit 6 precursor messenger RNA (pre-mRNA) by addition of 447 uridines and removal of 28 uridines. In vivo, the guide RNA gA6[14] is thought to specify the deletion of two uridines from the editing site closest to the 3' end. In this study, an in vitro system was developed that accurately removed uridines from this editing site in synthetic ATPase 6 pre-mRNA when gA6[14] and ATP were added. Mutations in both the guide RNA and the pre-mRNA editing site suggest that base-pairing interactions control the number of uridines deleted in vitro. Thus, guide RNAs are required for RNA editing and for the transfer of genetic information to pre-mRNAs.
布氏锥虫线粒体中的RNA编辑通过添加447个尿苷和去除28个尿苷，广泛改变了三磷酸腺苷合酶（ATPase）亚基6前体信使RNA（pre-mRNA）。在体内，引导RNA gA6[14]被认为指定从最靠近3'端的编辑位点删除两个尿苷。在本研究中，开发了一种体外系统，当添加gA6[14]和ATP时，该系统能准确地从合成的ATPase 6 pre-mRNA的这个编辑位点去除尿苷。引导RNA和pre-mRNA编辑位点的突变表明，碱基配对相互作用控制着体外删除的尿苷数量。因此，引导RNA对于RNA编辑以及将遗传信息转移到pre-mRNA是必需的。
