Savona C, Keramidas M, Chambaz E M, Feige J J
INSERM Unité 244, CEA, Biochemie des Régulations Cellulaires Endocrines, DBMS, CEN/G, Grenoble, France.
Growth Factors. 1994;10(3):197-205. doi: 10.3109/08977199409000238.
We report here that basic fibroblast growth factor (FGF-2), a potent mitogen for adrenocortical cells, stimulates the expression of alpha 2-macroglobulin by these cells at a transcriptional level and is synergistic with TGF beta 1 for this effect. This is supported by the following observations: (i) Treatment of adrenocortical cells by FGF-2 resulted in a time-dependent and dose-dependent increase of alpha 2M synthesis, (ii) FGF-2 did not modify alpha 2M secretion rate; (iii) The induction of alpha 2M synthesis by FGF-2 was not observed in the presence of the transcription inhibitor DRB; (iv) The amount of alpha 2M mRNA was increased by 2 to 3 fold under either FGF-2 or TGF beta 1 treatment; (v) Optimal doses of TGF beta and FGF-2 synergistically increased alpha 2M synthesis. Since alpha 2M is a growth factor-binding protein, its regulation by FGF-2 may represent an important feedback mechanism controlling the bioactivity of autocrine regulators (FGF-2, TFG beta) of adrenocortical functions.
我们在此报告，碱性成纤维细胞生长因子（FGF - 2）是肾上腺皮质细胞的一种强效促有丝分裂原，它在转录水平上刺激这些细胞表达α2 - 巨球蛋白，并且在此效应上与转化生长因子β1（TGFβ1）具有协同作用。以下观察结果支持了这一点：（i）用FGF - 2处理肾上腺皮质细胞导致α2M合成呈时间和剂量依赖性增加；（ii）FGF - 2未改变α2M分泌率；（iii）在存在转录抑制剂DRB的情况下未观察到FGF - 2诱导α2M合成；（iv）在FGF - 2或TGFβ1处理下，α2M mRNA的量增加了2至3倍；（v）TGFβ和FGF - 2的最佳剂量协同增加α2M合成。由于α2M是一种生长因子结合蛋白，其受FGF - 2的调节可能代表了一种控制肾上腺皮质功能自分泌调节因子（FGF - 2、TFGβ）生物活性的重要反馈机制。
