Yao Z J, Kao M C, Loh K C, Chung M C
Bioprocessing Technology Unit, National University of Singapore.
J Chromatogr A. 1994 Sep 9;679(1):190-4. doi: 10.1016/0021-9673(94)80326-9.
A peptide that contained one of the continuous epitopes of recombinant human lymphotoxin (rhLT) (amino acid residues 139-154) has been located by epitope mapping. The branched form of this peptide was synthesized by the multiple antigen peptide procedure with an octameric branched resin and was subsequently used to elicit anti-epitope antibody in rabbits. The resulting anti-epitope was then used as an immunoaffinity ligand in affinity chromatography to purify the parent protein, rhLT, from the host cell lysate directly. It is suggested that this approach would be a general way to create novel biospecific ligands for affinity separations.
通过表位作图确定了一种包含重组人淋巴毒素（rhLT）连续表位之一（氨基酸残基139 - 154）的肽。该肽的分支形式通过多抗原肽方法用八聚体分支树脂合成，随后用于在兔体内引发抗表位抗体。然后将所得的抗表位用作亲和色谱中的免疫亲和配体，直接从宿主细胞裂解物中纯化亲本蛋白rhLT。有人提出，这种方法将是创建用于亲和分离的新型生物特异性配体的通用方法。
