Villar M J, Ceccatelli S, Bedecs K, Bartfai T, Bredt D, Synder S H, Hökfelt T
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Brain Res. 1994 Jul 11;650(2):219-28. doi: 10.1016/0006-8993(94)91785-x.
The expression of several bioactive molecules in magnocellular hypothalamic neurons is modified when the axons of these cells are transected. In this study we have evaluated by means of immunocytochemistry and in situ hybridization the effect of hypophysectomy on the expression of nitric oxide synthase (NOS)- and of galanin message-associated peptide (GMAP)-like immunoreactivities (-LIs) as well as on their respective mRNAs in hypothalamic magnocellular neurosecretory neurons. The results show a transient increase in NOS- and GMAP-LIs in magnocellular neurons of both the paraventricular and supraoptic nuclei when compared to normal animals. The maximal increase in staining was observed between 5 and 7 days, and by 14 days NOS-LI was back to normal levels, whereas strong GMAP-LI could still be detected in a few cells. A similar picture was observed for the NOS and GMAP mRNAs. The functional significance of the present findings is unclear, but they indicate a possible role of nitric oxide and GMAP in neurosecretory neurons after injury.
当这些细胞的轴突被横断时，下丘脑大细胞神经元中几种生物活性分子的表达会发生改变。在本研究中，我们通过免疫细胞化学和原位杂交技术评估了垂体切除对下丘脑大细胞神经分泌神经元中一氧化氮合酶（NOS）和甘丙肽信息相关肽（GMAP）样免疫反应性（-LIs）及其各自mRNA表达的影响。结果显示，与正常动物相比，室旁核和视上核的大细胞神经元中NOS-和GMAP-LIs出现短暂增加。在第5至7天观察到染色增加最为明显，到第14天时，NOS-LI恢复到正常水平，而在少数细胞中仍可检测到较强的GMAP-LI。NOS和GMAP mRNA也出现了类似的情况。目前这些发现的功能意义尚不清楚，但它们表明一氧化氮和GMAP在损伤后的神经分泌神经元中可能发挥作用。
