Purkayastha R P, Mitra A K, Bhattacharyya B
Department of Botany, University of Calcutta, India.
Ecotoxicol Environ Saf. 1994 Feb;27(1):7-13. doi: 10.1006/eesa.1994.1002.
The uptake of heavy metals at sublethal concentrations by the mycelia and sporocarps of an edible fungus Pleurotus sajor-caju was measured by an atomic absorption spectrophotometer, and their impact on growth, productivity, and cellular proteins was also studied. Mycelia demonstrated the lowest uptake of Co2+ (11 micrograms ml-1) and Hg2+ (12 micrograms ml-1) and the highest uptake of Cu2+ (182 micrograms ml-1) and Cd2+ (178 micrograms ml-1). Sporocarps obtained from the substrate treated separately with Pb2+ (100 micrograms ml-1) and CD2+ (6 micrograms ml-1) indicated a minimum and maximum uptake of Pb2+ (7 micrograms g-1) and Cd2+ (33 micrograms g-1), respectively. Although Cu2+ and Cd2+ at 6 micrograms ml-1 indicated 41 and 93% growth reduction, Pb2+ and Hg2+ also caused more than 85% reduction of growth at 15 and 6 micrograms ml-1, respectively. Pb2+ reduced mycelial protein significantly (36%), but Hg2+ caused maximum reduction (30%) of proteins in sporocarps. Separate treatment of spawned substrate with test heavy metals reduced biological efficiency of sporocarp production, but markedly with Pb2+.
采用原子吸收分光光度计测定了亚致死浓度下重金属被食用菌佛罗里达侧耳的菌丝体和子实体的吸收情况，并研究了它们对生长、产量和细胞蛋白质的影响。菌丝体对Co2+（11微克/毫升）和Hg2+（12微克/毫升）的吸收最低，对Cu2+（182微克/毫升）和Cd2+（178微克/毫升）的吸收最高。分别用Pb2+（100微克/毫升）和Cd2+（6微克/毫升）处理底物后获得的子实体表明，Pb2+（7微克/克）的吸收最低，Cd2+（33微克/克）的吸收最高。尽管6微克/毫升的Cu2+和Cd2+分别使生长降低了41%和93%，但Pb2+和Hg2+在15微克/毫升和6微克/毫升时也分别使生长降低了85%以上。Pb2+显著降低了菌丝体蛋白质（36%），但Hg2+使子实体中的蛋白质降低最多（30%）。用测试重金属单独处理接种后的底物会降低子实体生产的生物学效率，但Pb2+的影响尤为明显。
