Grimes R W, Guthrie H D, Hammond J M
Department of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Endocrinology. 1994 Nov;135(5):1996-2000. doi: 10.1210/endo.135.5.7525259.
We compared insulin-like growth factor-binding protein (IGFBP) levels with indicators of follicular maturation and atresia in individual follicles of the porcine ovary. Follicular development was synchronized with the progestin, altrenogest, and progestin withdrawal was used to initiate the growth of an ovulatory cohort of follicles, which is accompanied by atresia of noncohort follicles. Individual follicles were isolated on days 1, 3, 5, and 7 after progestin withdrawal. Atretic follicles were identified by the presence of low hypodiploid levels of DNA in 10% or more of their granulosa cells using flow cytometry. The follicular fluid (FF) level of IGFBP-3 did not differ significantly between healthy and atretic medium-sized (3- to 6-mm) follicles and was not significantly correlated with the percentage of granulosa cells containing hypodiploid levels of DNA (r = 0.181) or with endocrine parameters such as FF concentrations of estradiol or androstenedione. However, among healthy follicles (atretic follicles removed from analyses to better examine follicular maturation), IGFBP-3 increased (P < 0.01) between days 1 and 7 and was positively correlated with follicle diameter (r = 0.514; P < 0.05) and the FF concentration of progesterone (r = 0.556; P < 0.01), indicators of the degree of follicular maturation. FF IGFBP-2 levels were 3-fold greater (P < 0.01) in atretic than in healthy follicles, and IGFBP-2 was correlated with percentage of granulosa cells containing hypodiploid levels of DNA (r = 0.729; P < 0.001). Among healthy follicles, FF IGFBP-2 did not differ significantly among days and was not significantly correlated with follicle diameter. These data suggest that the content of IGFBP-2 is related to the state of follicular health/atresia, whereas IGFBP-3 is related to preovulatory follicular development.
我们比较了猪卵巢单个卵泡中胰岛素样生长因子结合蛋白（IGFBP）水平与卵泡成熟和闭锁指标。卵泡发育通过孕激素炔诺孕酮同步化，孕激素撤回来启动排卵卵泡群的生长，同时伴随着非卵泡群卵泡的闭锁。在孕激素撤药后的第1、3、5和7天分离单个卵泡。使用流式细胞术，通过10%或更多颗粒细胞中低亚二倍体DNA水平的存在来鉴定闭锁卵泡。健康和闭锁的中等大小（3至6毫米）卵泡之间，卵泡液（FF）中IGFBP-3水平无显著差异，且与含有亚二倍体DNA水平的颗粒细胞百分比（r = 0.181）或内分泌参数如FF中雌二醇或雄烯二酮浓度无显著相关性。然而，在健康卵泡中（为更好地检查卵泡成熟，从分析中去除闭锁卵泡），IGFBP-3在第1天至第7天之间升高（P < 0.01），且与卵泡直径（r = 0.514；P < 0.05）和FF中孕酮浓度（r = 0.556；P < 0.01）呈正相关，这两个指标是卵泡成熟程度的指标。闭锁卵泡中FF的IGFBP-2水平比健康卵泡高3倍（P < 0.01），且IGFBP-2与含有亚二倍体DNA水平的颗粒细胞百分比相关（r = 0.729；P < 0.001）。在健康卵泡中，FF的IGFBP-2在各天之间无显著差异，且与卵泡直径无显著相关性。这些数据表明，IGFBP-2的含量与卵泡健康/闭锁状态有关，而IGFBP-3与排卵前卵泡发育有关。
