在急性胰腺炎大鼠模型中，酶原颗粒蛋白GP2的水平升高。

The level of the zymogen granule protein GP2 is elevated in a rat model for acute pancreatitis.

作者信息

Lowe A W, Luthen R E, Wong S M, Grendell J H

机构信息

Department of Medicine, Stanford University School of Medicine, California.

出版信息

Gastroenterology. 1994 Dec;107(6):1819-27. doi: 10.1016/0016-5085(94)90826-5.

DOI:10.1016/0016-5085(94)90826-5

PMID:7525398

Abstract

BACKGROUND/AIMS: GP2 is the major membrane protein in pancreatic zymogen granules. It is linked to the membrane via a glycosyl-phosphatidylinositol linkage. After cleavage, a significant fraction of GP2 becomes soluble. The present study assessed whether GP2 is a useful serum marker for acute pancreatitis.

METHODS

Using an anti-GP2 monoclonal antibody, an enzyme-linked immunosorbent assay was developed to measure the serum levels of GP2 in rats with cerulein-induced acute pancreatitis.

RESULTS

The anti-GP2 antibody was specific because it did not cross-react with uromodulin, a structurally similar protein to GP2, or to protein extracts from nonpancreatic tissues. Eight hours after the induction of pancreatitis, the serum levels of amylase, lipase, and GP2 peaked. Peak GP2 levels were 4.2 times higher than those of controls. At 24 hours, GP2 was still 70% of the peak level, whereas amylase and lipase were 5.5% and 0.5%, respectively, of their peak levels.

CONCLUSIONS

GP2 may serve as a potentially valuable marker for clinical acute pancreatitis.

摘要

背景/目的：GP2是胰腺酶原颗粒中的主要膜蛋白。它通过糖基磷脂酰肌醇连接与膜相连。切割后，相当一部分GP2变得可溶。本研究评估GP2是否为急性胰腺炎的有用血清标志物。

方法

使用抗GP2单克隆抗体，开发了一种酶联免疫吸附测定法来测量用雨蛙肽诱导的急性胰腺炎大鼠的血清GP2水平。

结果

抗GP2抗体具有特异性，因为它不与尿调节蛋白（一种结构与GP2相似的蛋白质）或非胰腺组织的蛋白质提取物发生交叉反应。胰腺炎诱导后8小时，淀粉酶、脂肪酶和GP2的血清水平达到峰值。GP2峰值水平比对照组高4.2倍。在24小时时，GP2仍为峰值水平的70%，而淀粉酶和脂肪酶分别为其峰值水平的5.5%和0.5%。