Landemore G, Quillec M, Letaïef S E, Izard J
Laboratoire d'Histologie, UFR de Médecine, Université de Caen, France.
Histochem J. 1994 Jul;26(7):571-81. doi: 10.1007/BF00158591.
This study deals with the ultrastructure of the chondroitin sulphate proteoglycans of the Kurloff body, a large lysosome organelle, metachromatic towards Toluidine Blue, of a blood cell unique to the guinea pig and called the Kurloff cell. Splenic Kurloff cell from oestrogen-treated guinea pig cells were examined after staining with Cuprolinic Blue, a cationic phthalocyanine-like dye, in the presence of MgCl2 in a critical electrolyte concentration method. Better results were obtained when the fixation-staining by the glutaraldehyde Cuprinolinic Blue MgCl2 mixture was preceded by a glutaraldehyde pre-fixation. On light microscopy, Kurloff bodies generally exhibited an overall pink and glassy metachromasia, sometimes with additional darker metachromatic small dots at their peripheries. At the ultrastructural level, the metachromatic central matrix of the Kurloff body usually exhibited, as a major feature, a typical network pattern of ribbon-like or stellate electron-dense precipitates suggesting the presence of a skeleton of Cuprolinic Blue-reactive filamentous structures. Taking into account their high anionicity (as shown by the stability of the dye binding in the presence of 0.3 M MgCl2) and their susceptibility to chondroitinase ABC, these anionic structures were assumed to be related to the proteochondroitin-4-sulphate previously characterized as the only major sulphated glycoconjugate of the Kurloff cell.
本研究探讨了库尔洛夫小体的硫酸软骨素蛋白聚糖的超微结构。库尔洛夫小体是一种大型溶酶体细胞器，对甲苯胺蓝呈异染性，存在于豚鼠特有的一种血细胞——库尔洛夫细胞中。采用临界电解质浓度法，在氯化镁存在的情况下，用阳离子酞菁类染料铜啉蓝对经雌激素处理的豚鼠脾脏库尔洛夫细胞进行染色后进行检查。若先用戊二醛预固定，再用戊二醛 - 铜啉蓝 - 氯化镁混合物进行固定染色，则能获得更好的结果。在光学显微镜下，库尔洛夫小体通常呈现出整体粉红色且似玻璃样的异染性，有时在其周边还会有额外较深的异染性小点。在超微结构水平上，库尔洛夫小体的异染性中央基质通常呈现出一种主要特征，即典型的带状或星状电子致密沉淀物网络模式，这表明存在与铜啉蓝反应性丝状结构的骨架。考虑到它们的高阴离子性（如在0.3 M氯化镁存在下染料结合的稳定性所示）以及它们对软骨素酶ABC的敏感性，这些阴离子结构被认为与先前被鉴定为库尔洛夫细胞唯一主要硫酸化糖缀合物的蛋白聚糖 - 4 - 硫酸盐有关。
