Batch J A, Mercuri F A, Edmondson S R, Werther G A
Center for Hormone Research, University of Melbourne, Royal Children's Hospital, Parkville, Australia.
J Clin Endocrinol Metab. 1994 Nov;79(5):1444-9. doi: 10.1210/jcem.79.5.7525637.
The role of the insulin-like growth factors (IGFs) in human skin physiology has been increasingly recognized, although relatively little is known about the cell types involved or the cellular mechanisms that mediate these responses. Epidermal keratinocytes and dermal fibroblasts both possess IGF-I receptors and are responsive to IGF-I. IGF-binding proteins (IGFBPs), known modulators of IGF action, may also be responsible for targeting IGF-I to its receptors and are produced by both cultured keratinocytes and fibroblasts. To demonstrate sites of production of IGFBPs in human skin, we have used in situ hybridization to localize messenger ribonucleic acid (mRNA) for the six IGFBPs. Antisense and sense RNA probes for the IGFBPs (IGFBP-1 to -6) were produced, and 5-microns sections of normal adult human male chest skin were probed. The control probe used was keratin-5, which is known to hybridize to the basal keratinocytes of the epidermis. mRNAs for human IGFBP-2, -3, -4, and -5 were identified, with mRNAs for IGFBP-2 and IGFBP-4 localized in sebaceous glands and eccrine sweat glands (epidermal origin), IGFBP-3 mRNA in the basal layer of the epidermis and mRNAs for IGFBP-4, and IGFBP-5 found throughout the dermis. mRNAs for IGFBP-1 and -6 were not identified in human skin. These studies demonstrate specific localization of IGFBP mRNAs in adult human skin, suggesting that each IGFBP may play a specific role in targeting IGF-I to its receptor on responsive cells and, ultimately, in modulation of IGF-I action in skin.
胰岛素样生长因子（IGFs）在人类皮肤生理学中的作用已得到越来越多的认可，尽管对于所涉及的细胞类型或介导这些反应的细胞机制了解相对较少。表皮角质形成细胞和真皮成纤维细胞都拥有IGF-I受体，并且对IGF-I有反应。IGF结合蛋白（IGFBPs）是已知的IGF作用调节剂，也可能负责将IGF-I靶向其受体，并且由培养的角质形成细胞和成纤维细胞产生。为了证明IGFBPs在人类皮肤中的产生部位，我们使用原位杂交来定位六种IGFBPs的信使核糖核酸（mRNA）。制备了针对IGFBPs（IGFBP-1至-6）的反义RNA探针和正义RNA探针，并对正常成年男性胸部皮肤的5微米切片进行检测。所使用的对照探针是角蛋白-5，已知它可与表皮的基底角质形成细胞杂交。已鉴定出人类IGFBP-2、-3、-4和-5的mRNA，其中IGFBP-2和IGFBP-4的mRNA定位于皮脂腺和小汗腺（表皮来源），IGFBP-3的mRNA定位于表皮的基底层，而IGFBP-4和IGFBP-5的mRNA在整个真皮中均有发现。在人类皮肤中未鉴定出IGFBP-1和-6的mRNA。这些研究证明了IGFBP mRNA在成年人类皮肤中的特定定位，表明每种IGFBP可能在将IGF-I靶向其反应性细胞上的受体以及最终调节皮肤中IGF-I的作用方面发挥特定作用。
