Edyvane K A, Smet P J, Trussell D C, Jonavicius J, Marshall V R
Research Laboratories, Repatriation General Hospital, Daw Park, South Australia.
J Auton Nerv Syst. 1994 Aug;48(3):241-55. doi: 10.1016/0165-1838(94)90053-1.
The patterns of colocalisation of neuropeptides, tyrosine hydroxylase (TH), and protein gene product 9.5 (PGP), were studied in nerve fibres supplying the upper and lower human ureter using a double labelling immunofluorescence technique. The majority (85%-95%) of nerve fibres within the ureter contained neuropeptide Y-like immunoreactivity (NPY-LIR), in combination with other peptides. Approximately 52%-63% of the total ureteral innervation was made up of NPY-LIR fibres also expressing TH-LIR, while 21%-42% of fibres contained NPY-LIR in combination with vasoactive intestinal polypeptide (VIP)-LIR. These two immunochemically defined classes did not overlap, since TH- and VIP-LIR were never present within the same nerve fibre. Other minor populations of neurones included those containing calcitonin gene-related peptide (CGRP)-LIR in combination with substance P (SP)-LIR (4%-17%) and those without SP (5%). Rare coexistences were also noted between CGRP- and VIP-LIR (1%-2%), CGRP- and NPY-LIR (< or = 1%), and CGRP- and TH-LIR (< 1%). Regional differences in innervation were found. There were fewer of each class of nerve fibres in the upper ureter compared to the lower ureter. In addition, the proportion of VIP/NPY-LIR fibres of the total innervation was less in the upper ureter, where they were very sparse. Differences in the distribution to various tissue targets were also observed. In the lower ureter, TH/NPY-LIR fibres were localised predominantly to the outer muscle fascicles and adventitia, while VIP/NPY immunoreactive nerves supplied the submucosa and inner smooth muscle fascicles. Both of these populations were also found around blood vessels. A population of presumptive sensory fibres expressing CGRP/SP-LIR were typically present immediately beneath the urinary epithelium and around blood vessels, and only very rarely within muscle fascicles. The finding that TH/NPY- and VIP/NPY-LIR fibres innervate different layers of the ureter raises the possibility that the muscle layers of the ureter may be independently controlled.
利用双标记免疫荧光技术，研究了供应人输尿管上段和下段的神经纤维中神经肽、酪氨酸羟化酶（TH）和蛋白基因产物9.5（PGP）的共定位模式。输尿管内的大多数神经纤维（85%-95%）含有神经肽Y样免疫反应性（NPY-LIR），并与其他肽类结合。输尿管总神经支配中约52%-63%由同时表达TH-LIR的NPY-LIR纤维组成，而21%-42%的纤维含有与血管活性肠肽（VIP）-LIR结合的NPY-LIR。这两类免疫化学定义的神经纤维不重叠，因为TH-LIR和VIP-LIR从未出现在同一神经纤维内。其他少量神经元群体包括含有降钙素基因相关肽（CGRP）-LIR与P物质（SP）-LIR结合的神经元（4%-17%）以及不含SP的神经元（5%）。还发现CGRP-LIR与VIP-LIR（1%-2%）、CGRP-LIR与NPY-LIR（≤1%）以及CGRP-LIR与TH-LIR（<1%）之间存在罕见的共存情况。发现了神经支配的区域差异。与输尿管下段相比，输尿管上段各类神经纤维数量较少。此外，输尿管上段总神经支配中VIP/NPY-LIR纤维的比例较低，且分布非常稀疏。还观察到向不同组织靶点分布的差异。在输尿管下段，TH/NPY-LIR纤维主要定位于外肌束和外膜，而VIP/NPY免疫反应性神经供应黏膜下层和内平滑肌束。这两类神经纤维在血管周围也均有发现。一群表达CGRP/SP-LIR的假定感觉纤维通常位于尿上皮下方和血管周围，仅极少出现在肌束内。TH/NPY-LIR和VIP/NPY-LIR纤维支配输尿管不同层的这一发现增加了输尿管肌层可能受到独立控制的可能性。
