Palmer R M, Loveridge N, Thomson B M, Mackie S C, Tonner E, Flint D J
Rowett Research Institute, Bucksburn, Aberdeen, UK.
J Endocrinol. 1994 Jul;142(1):85-91. doi: 10.1677/joe.0.1420085.
A polyclonal antiserum to rat GH (anti-rGH) injected into rats for 3 or 8 weeks markedly reduced the weight, total protein and RNA content of muscles of the hind limb. These effects were prevented when bovine GH (bGH) was administered simultaneously. In a second experiment, the effects of 8 weeks of treatment with anti-rGH on the growth of the whole body, muscle and bone were investigated. Body weights of rats were decreased by 58% by treatment with anti-rGH; muscle weights were reduced by slightly more than the decrease in body weight (by 64%, 65% and 61% respectively for plantaris, soleus and gastrocnemius). The weight of the tibia was decreased by 54%, its length was decreased by 23%, cortical width and overall width were reduced by 26% and 18% respectively, suggesting a possible role for GH in osteoclastic activity. Serum total insulin-like growth factor-I (IGF-I) concentrations were decreased by 80-90% in both experiments by anti-rGH; these changes were prevented in the first experiment by concurrent treatment with anti-rGH and bGH. The serum IGF-binding protein-3 (IGFBP-3) concentration was also decreased by anti-rGH in experiment 1 (by 86%); the response of the 28-32 kDa IGFBPs was smaller (-35%), and was restored to control values by simultaneous injection of bGH. Western immunoblotting using an antiserum to IGFBP-2 showed that there was a marked decrease from neonatal to adult stages which was independent of anti-rGH treatment. This clearly demonstrated a dissociation of the reciprocal relationship supposed to exist between IGFBPs-2 and -3. The 24 kDa IGFBP-4 was unaffected by anti-rGH but replacement therapy with bGH doubled its concentration. Although the effects on body and muscle weight were prevented when rats were given anti-rGH and bGH simultaneously, the possibility of mediation by other hormones cannot be precluded.
给大鼠注射抗大鼠生长激素（anti-rGH）3周或8周，显著降低了后肢肌肉的重量、总蛋白和RNA含量。同时给予牛生长激素（bGH）可防止这些作用。在第二个实验中，研究了anti-rGH治疗8周对全身、肌肉和骨骼生长的影响。用anti-rGH治疗使大鼠体重降低了58%；肌肉重量的降低略高于体重的降低（比目鱼肌、比目鱼肌和腓肠肌分别降低了64%、65%和61%）。胫骨重量降低了54%，长度降低了23%，皮质宽度和整体宽度分别降低了26%和18%，提示生长激素在破骨细胞活性中可能起作用。在两个实验中，anti-rGH均使血清总胰岛素样生长因子-I（IGF-I）浓度降低了80-90%；在第一个实验中，同时给予anti-rGH和bGH可防止这些变化。在实验1中，anti-rGH也降低了血清IGF结合蛋白-3（IGFBP-3）浓度（降低了86%）；28-32 kDa IGFBPs的反应较小（降低了35%），同时注射bGH可使其恢复到对照值。使用抗IGFBP-2抗血清进行的Western免疫印迹显示，从新生期到成年期有明显下降，且与anti-rGH治疗无关。这清楚地证明了IGFBP-2和-3之间本应存在的相互关系的解离。24 kDa IGFBP-4不受anti-rGH影响，但用bGH替代疗法可使其浓度增加一倍。尽管同时给予anti-rGH和bGH可防止对体重和肌肉重量的影响，但不能排除其他激素介导的可能性。
