Phang T W, Shi C Y, Chia J N, Ong C N
Department of Community, Occupational and Family Medicine, Singapore.
J Forensic Sci. 1994 Sep;39(5):1275-9.
Analysis of cDNA derived from messenger RNA is of advantage over using genomic DNA in genetic analysis of large genes, especially those with lengthy intron sequences. However, because of its instability and rapid degradation, RNA extraction from postmortem tissues has not been attempted. Here, we report the successful extraction of intact mRNA from various postmortem tissues from accidental and sudden death cases. Subsequently with reverse transcriptase-polymerase chain reaction (RT-PCR), we were able to amplify cDNA fragments of different lengths up to 0.9 kb. The described method therefore provides a useful tool in genetic analysis of postmortem tissues.
在大型基因的遗传分析中，尤其是那些具有冗长内含子序列的基因，对源自信使核糖核酸的互补脱氧核糖核酸进行分析比使用基因组脱氧核糖核酸更具优势。然而，由于核糖核酸的不稳定性和快速降解，尚未尝试从死后组织中提取核糖核酸。在此，我们报告了从意外死亡和猝死病例的各种死后组织中成功提取完整信使核糖核酸的方法。随后，通过逆转录聚合酶链反应（RT-PCR），我们能够扩增出长度不同、最长达0.9千碱基的互补脱氧核糖核酸片段。因此，所描述的方法为死后组织的遗传分析提供了一个有用的工具。
