Kurihara J, Hu B R, Wieloch T
Laboratory for Experimental Brain Research, Lund University Hospital, Sweden.
J Neurochem. 1994 Dec;63(6):2346-8. doi: 10.1046/j.1471-4159.1994.63062346.x.
The changes in the levels of tyrosine-phosphorylated proteins in the cytosolic fraction of the rat hippocampus subjected to severe hypoglycemia were analyzed. A marked increase in tyrosine phosphorylation of a 43-kDa protein was observed at 30 min of isoelectric EEG and 30 min and 1 h of recovery. Immunostaining of the same blot with antibody against mitogen-activated protein (MAP) kinase demonstrated a double band of approximately 42 and 43 kDa. The increased tyrosine phosphorylation of MAP kinase during hypoglycemic coma and the early recovery period suggests that MAP kinase may be involved in neuronal degeneration and repair.
分析了经历严重低血糖的大鼠海马细胞质部分中酪氨酸磷酸化蛋白水平的变化。在等电位脑电图30分钟以及恢复30分钟和1小时时，观察到一种43 kDa蛋白的酪氨酸磷酸化显著增加。用抗丝裂原活化蛋白（MAP）激酶抗体对同一印迹进行免疫染色，显示出约42和43 kDa的两条带。低血糖昏迷和早期恢复期间MAP激酶酪氨酸磷酸化的增加表明，MAP激酶可能参与神经元变性和修复。
