一种提高示踪物质离子导入给药可靠性的简单方法。

A simple method to improve the reliability of iontophoretic administration of tracer substances.

作者信息

Yu D, Gordon F J

机构信息

Department of Pharmacology, Rollins Research Center, Emory University School of Medicine, Atlanta, GA 30322.

出版信息

J Neurosci Methods. 1994 Jun;52(2):161-4. doi: 10.1016/0165-0270(94)90125-2.

DOI:10.1016/0165-0270(94)90125-2

PMID:7526084

Abstract

Microiontophoresis is a widely used technique for depositing tracer materials into the central nervous system for neuroanatomical experiments. However, the reliability of iontophoretic injection is often less than optimal. Coating the lumen of glass micropipettes with silicone to reduce surface tension reduced the incidence of failure for iontophoretic deposition of wheat germ agglutinin-horseradish peroxidase (WGA-HRP) from 40% to zero. Similar results were observed with other tracer substances. Siliconizing the internal surface of glass micropipettes can significantly improve the reliability of iontophoretic deposition.

摘要

微离子电渗疗法是一种广泛应用于神经解剖学实验中，将示踪物质注入中枢神经系统的技术。然而，离子电渗注射的可靠性往往不尽如人意。用硅酮涂层玻璃微吸管内腔以降低表面张力，可将小麦胚芽凝集素-辣根过氧化物酶（WGA-HRP）离子电渗沉积的失败率从40%降至零。其他示踪物质也观察到了类似结果。对玻璃微吸管内表面进行硅化处理可显著提高离子电渗沉积的可靠性。