从大鼠大脑分离的培养星形胶质细胞中组成型一氧化氮合酶的存在。

Presence of constitutive type nitric oxide synthase in cultured astrocytes isolated from rat cerebra.

作者信息

Ma L, Morita I, Murota S

机构信息

Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.

出版信息

Neurosci Lett. 1994 Jun 20;174(2):123-6. doi: 10.1016/0304-3940(94)90001-9.

DOI:10.1016/0304-3940(94)90001-9

PMID:7526282

Abstract

To define whether astrocytes express a constitutive nitric oxide synthase (NOS), nitric oxide (NO) producing activity in astrocytes derived fetal rat cerebra was examined. We found that the addition of an NOS inhibitor to cultures caused decrease in the basal cGMP levels in unstimulated astrocytes and the decrease was dose-dependent. Further, the expression of cNOS activity in unstimulated astrocytes was confirmed by histochemical staining for NADPH diaphorase and by measuring conversion of L-arginine to L-citrulline. We conclude that cultured astrocytes express constitutive NOS, in addition to inducible one.

摘要

为了确定星形胶质细胞是否表达组成型一氧化氮合酶（NOS），我们检测了源自胎鼠大脑的星形胶质细胞中一氧化氮（NO）的产生活性。我们发现，向培养物中添加NOS抑制剂会导致未受刺激的星形胶质细胞中基础环磷酸鸟苷（cGMP）水平降低，且这种降低呈剂量依赖性。此外，通过对NADPH黄递酶进行组织化学染色以及测量L-精氨酸向L-瓜氨酸的转化，证实了未受刺激的星形胶质细胞中cNOS活性的表达。我们得出结论，培养的星形胶质细胞除了表达诱导型NOS外，还表达组成型NOS。