雌激素与日本鹌鹑肾细胞核的特异性结合。

Specific binding of estrogen to nuclei from Japanese quail kidney cells.

作者信息

Turner R T, Howard G A, Baylink D J

出版信息

Endocr Res Commun. 1978;5(4):335-42. doi: 10.1080/07435807809061097.

Abstract

The binding properties of estrogen to Japanese quail kidney cell nuclei were evaluated by 3H-17 beta-estradiol exchange. Kidney cell nuclei from estrogenized male, as well as laying female quail, had a single class of saturable (0.1-0.2 pmol/mg DNA), high affinity (Kd = 1-2 x 10-10M) nuclear binding sites for 17 beta-estradiol. High affinity estrogen binding sites were not found in kidney cell nuclei from untreated males. Various non-radioactive estrogens (17 beta-estradiol, estrone, estriol and diethylstilbestrol) competed effectively with 3H-17 beta-estradiol for the high affinity binding sites, whereas nonestrogens (testosterone and progesterone) did not complete. It is concluded that the 3H-estradiol binding characteristics of kidney cell nuclei provides strong evidence for a nuclear estrogen receptor in the avian kidney.

摘要

通过³H-17β-雌二醇交换评估雌激素与日本鹌鹑肾细胞核的结合特性。来自经雌激素处理的雄性以及产蛋雌性鹌鹑的肾细胞核，对于17β-雌二醇具有一类可饱和的（0.1 - 0.2 pmol/mg DNA）、高亲和力（Kd = 1 - 2×10⁻¹⁰M）的核结合位点。在未处理雄性的肾细胞核中未发现高亲和力雌激素结合位点。各种非放射性雌激素（17β-雌二醇、雌酮、雌三醇和己烯雌酚）能有效地与³H-17β-雌二醇竞争高亲和力结合位点，而非雌激素（睾酮和孕酮）则不能竞争。结论是，肾细胞核的³H-雌二醇结合特性为禽类肾脏中的核雌激素受体提供了有力证据。