Gosling P, Zareian M
Department of Clinical Biochemistry, Selly Oak Hospital, Birmingham, UK.
Ann Clin Biochem. 1994 Jul;31 ( Pt 4):371-3. doi: 10.1177/000456329403100412.
Two commercial amylase methods which employ p-nitrophenol derivatives of blocked maltoheptaosides suffer from negative interference due to fresh haemolysate. Specimen storage at room temperature, or pre-incubation at 37 degrees C or 57 degrees C removes the effect. Incubation of amylase reagent at 37 degrees C with fresh haemolysate where the final haemoglobin concentration was 0.011 g/L, showed a rapid fall in absorption around 414 nm which became stable after 150 min. Since p-nitrophenol, the product of the amylase reaction, is measured at 405 nm it is concluded that the negative interference from fresh haemolysis is due to the transitory fall in absorption around 405 nm. It is recommended that amylase measurements using this technique, particularly those performed as an emergency, should not be done on haemolysed specimens.
两种使用封闭麦芽七糖的对硝基苯酚衍生物的商业淀粉酶检测方法，会因新鲜溶血产物而受到负干扰。将样本在室温下储存，或在37℃或57℃下预孵育可消除这种影响。在最终血红蛋白浓度为0.011 g/L的新鲜溶血产物存在的情况下，将淀粉酶试剂在37℃孵育，结果显示在414 nm左右的吸光度迅速下降，150分钟后趋于稳定。由于淀粉酶反应的产物对硝基苯酚是在405 nm处进行测量的，因此可以得出结论，新鲜溶血产生的负干扰是由于405 nm左右吸光度的短暂下降所致。建议使用该技术进行淀粉酶检测时，尤其是急诊检测，不应在溶血样本上进行。
