Aptel H B, Deacon C F, Henderson I W
Sheffield University, Department of Animal and Plant Sciences, Western Bank, England.
Biochem Biophys Res Commun. 1994 Nov 15;204(3):1286-91. doi: 10.1006/bbrc.1994.2602.
The interactions of Atrial Natriuretic Peptide with its receptors were investigated with the vascular smooth muscle cells from the A7r5 cell line. Displacement experiments of [125I]rat-Atrial Natriuretic Peptide by rat-Atrial Natriuretic Peptide revealed a single class of receptors with a Kd = 0.21 +/- 0.08 nM and a Bmax = of 35 +/- 16 fmol/mg of protein. Furthermore, the complex [125I]r-Atrial Natriuretic Peptide was internalised by a heatsensitive process. Finally, Atrial Natriuretic Peptide increased cyclic Guanosyl 3',5' Mono Phosphate in a time dependent and dose dependent way, a concentration of 0.1 microM increasing cyclic Guanosyl 3',5' Mono Phosphate level by a factor of 8.3 times when compared to basal level.
利用A7r5细胞系的血管平滑肌细胞研究了心房利钠肽与其受体的相互作用。大鼠心房利钠肽对[125I]大鼠心房利钠肽的置换实验显示存在一类单一的受体，其解离常数（Kd）=0.21±0.08 nM，最大结合容量（Bmax）为35±16 fmol/mg蛋白质。此外，[125I]r-心房利钠肽复合物通过一个热敏感过程被内化。最后，心房利钠肽以时间和剂量依赖的方式增加环磷酸鸟苷，与基础水平相比，0.1 microM的浓度可使环磷酸鸟苷水平增加8.3倍。
