Ahlén K, Rubin K
Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
Exp Cell Res. 1994 Dec;215(2):347-53. doi: 10.1006/excr.1994.1351.
Platelet-derived growth factor (PDGF)-BB stimulates fibroblast-mediated contraction of collagen gels, as well as migration of fibroblasts through collagen-coated membranes. In the present study we examined effects of PDGF-BB stimulation on the synthesis of collagen-binding beta 1 integrins by human diploid fibroblasts (AG 1518). PDGF-BB stimulation led to an increase in the rare of integrin alpha 2-subunit synthesis. In contrast, synthesis of the integrin alpha 1- or alpha 3-subunits were not affected by PDGF-BB stimulation. Furthermore, levels of alpha 2-subunit mRNA relative to levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA increased after PDGF-BB stimulation. The latter finding is compatible with PDGF-BB stimulating transcription of the alpha 2-subunit gene. PDGF-BB stimulation did not influence the relation between levels of integrin beta 1-subunit mRNA and GAPDH mRNA. In addition, the rate of synthesis or post-translational processing of the integrin beta 1-subunit were not, or only marginally, affected by PDGF-BB stimulation. It is likely that the motility response elicited in fibroblasts by PDGF-BB involves such alterations in the synthesis of the alpha 2-subunit of the alpha 2 beta 1 collagen-binding integrin.
血小板衍生生长因子（PDGF）-BB可刺激成纤维细胞介导的胶原凝胶收缩，以及成纤维细胞通过胶原包被膜的迁移。在本研究中，我们检测了PDGF-BB刺激对人二倍体成纤维细胞（AG 1518）胶原结合β1整合素合成的影响。PDGF-BB刺激导致整合素α2亚基合成速率增加。相比之下，整合素α1或α3亚基的合成不受PDGF-BB刺激的影响。此外，PDGF-BB刺激后，相对于甘油醛-3-磷酸脱氢酶（GAPDH）mRNA水平，α2亚基mRNA水平升高。后一发现与PDGF-BB刺激α2亚基基因转录一致。PDGF-BB刺激不影响整合素β1亚基mRNA水平与GAPDH mRNA水平之间的关系。此外，整合素β1亚基的合成速率或翻译后加工不受PDGF-BB刺激影响，或仅受到轻微影响。PDGF-BB在成纤维细胞中引发的运动反应可能涉及α2β1胶原结合整合素α2亚基合成的此类改变。
