Vuori K, Ruoslahti E
Cancer Research Center, La Jolla Cancer Research Foundation, CA 92037.
Science. 1994 Dec 2;266(5190):1576-8. doi: 10.1126/science.7527156.
Insulin stimulation was found to promote association of the alpha v beta 3 integrin (a vitronectin receptor) with insulin receptor substrate-1 (IRS-1), an intracellular protein that mediates insulin signaling by binding other signaling molecules, including growth factor receptor-bound protein 2 (Grb2) and phosphatidylinositol-3' kinase. Insulin-treated cells expressing the alpha v beta 3 integrin showed 2.5 times more DNA synthesis when plated on vitronectin than on other substrates, whereas cells expressing another vitronectin receptor, alpha v beta 5, did not show this difference. The association between integrin and IRS-1 may be a mechanism for the synergistic action of growth factor and extracellular matrix receptors.
研究发现，胰岛素刺激可促进αvβ3整合素（一种玻连蛋白受体）与胰岛素受体底物-1（IRS-1）结合，IRS-1是一种细胞内蛋白，通过与包括生长因子受体结合蛋白2（Grb2）和磷脂酰肌醇-3'激酶在内的其他信号分子结合来介导胰岛素信号传导。在玻连蛋白上培养时，表达αvβ3整合素的胰岛素处理细胞的DNA合成比在其他底物上培养时多2.5倍，而表达另一种玻连蛋白受体αvβ5的细胞则未表现出这种差异。整合素与IRS-1之间的关联可能是生长因子和细胞外基质受体协同作用的一种机制。
