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使用靶向16S rRNA的寡核苷酸探针研究瘤胃纤维分解菌之间的竞争:纤维素和碱性过氧化氢处理麦秸的纯培养研究

The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: pure-culture studies with cellulose and alkaline peroxide-treated wheat straw.

作者信息

Odenyo A A, Mackie R I, Stahl D A, White B A

机构信息

Department of Animal Sciences, University of Illinois at Urbana-Champaign 61801.

出版信息

Appl Environ Microbiol. 1994 Oct;60(10):3697-703. doi: 10.1128/aem.60.10.3697-3703.1994.

Abstract

Specific oligonucleotide probes targeted to sites on the 16S rRNA of Ruminococcus albus 8, Ruminococcus flavefaciens FD-1, and Fibrobacter succinogenes S85 and a domain Bacteria probe were used to study bacterial interactions during the fermentation of cellulose and alkaline hydrogen peroxide-treated wheat straw in monocultures, dicultures, and tricultures. Results showed that R. albus 8 inhibited the growth of R. flavefaciens FD-1 when grown as a diculture with cellulose or alkaline hydrogen peroxide-treated wheat straw as the carbon source. In dicultures containing R. albus 8 and F. succinogenes S85 grown on cellulose or alkaline hydrogen peroxide-treated wheat straw, competition was not detected. R. flavefaciens FD-1 outcompeted F. succinogenes S85 when cellulose was used as the carbon source. In tricultures with cellulose as the carbon source, R. flavefaciens FD-1 was inhibited, R. albus 8 appeared to dominate during the early phase of degradation (12 to 48 h), while F. succinogenes S85 became predominant during the later phase of degradation (60 to 70 h). When alkaline hydrogen peroxide-treated wheat straw was used as a growth substrate, F. succinogenes S85 showed better growth than either R. albus 8 or R. flavefaciens FD-1. However, R. flavefaciens FD-1 was present in small numbers throughout the incubation period, unlike the growth patterns when cellulose was the carbon source.

摘要

针对白色瘤胃球菌8、黄色瘤胃球菌FD-1和琥珀酸纤维杆菌S85的16S rRNA位点的特异性寡核苷酸探针以及一个细菌域探针,被用于研究在单培养、双培养和三培养中纤维素和碱性过氧化氢处理的小麦秸秆发酵过程中的细菌相互作用。结果表明,当以纤维素或碱性过氧化氢处理的小麦秸秆作为碳源进行双培养时,白色瘤胃球菌8抑制了黄色瘤胃球菌FD-1的生长。在以纤维素或碱性过氧化氢处理的小麦秸秆为生长底物的含有白色瘤胃球菌8和琥珀酸纤维杆菌S85的双培养中,未检测到竞争。当以纤维素作为碳源时,黄色瘤胃球菌FD-1胜过琥珀酸纤维杆菌S85。在以纤维素作为碳源的三培养中,黄色瘤胃球菌FD-1受到抑制,白色瘤胃球菌8在降解早期阶段(12至48小时)似乎占主导地位,而琥珀酸纤维杆菌S85在降解后期阶段(60至70小时)变得占优势。当以碱性过氧化氢处理的小麦秸秆作为生长底物时,琥珀酸纤维杆菌S85的生长比白色瘤胃球菌8或黄色瘤胃球菌FD-1都要好。然而,与以纤维素作为碳源时的生长模式不同,黄色瘤胃球菌FD-1在整个培养期间数量都很少。

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