Bechtol K B, Hanzel D K, Liang B C
Molecular Dynamics Inc., Sunnyvale, CA 94086.
Am Biotechnol Lab. 1994 Dec;12(13):8-10.
The FluorImager fluorescence imaging system uses monochromatic 488-nm laser light to excite fluorochromes. It contains a built-in 515-nm long-pass filter that rejects excitation laser light, but allows emission light with wavelengths longer than 515 nm to pass through. A fluorochrome appropriate for use in the system is excitable by 488-nm light and emits at least some of its fluorescence at wavelengths longer than 515 nm. Two types of optical filters, long-pass and band-pass, are used in the system. Long-pass filters reject shorter wavelengths and transmit longer wavelengths. The number in the filter name denotes the cutoff wavelength (midpoint of the transition between rejected and transmitted light) for the filter. Band-pass filters transmit a band of wavelengths and reject both shorter and longer wavelengths. The numbers in the filter name denote the center wavelength of the passed band and the width of the band at half maximum transmission. Generally, when scanning for a single fluorochrome, only the built-in 515-nm long-pass filter is needed. An interchangeable filter can be added to decrease the contribution from a broad-spectrum background signal and to attenuate strong fluorochrome signals.
荧光成像系统利用488纳米的单色激光激发荧光染料。它内置一个515纳米的长波通滤光片，可阻挡激发激光，但允许波长大于515纳米的发射光通过。适用于该系统的荧光染料可被488纳米的光激发，并在波长大于515纳米处发射至少部分荧光。该系统使用两种类型的光学滤光片，即长波通和带通滤光片。长波通滤光片阻挡较短波长并透射较长波长。滤光片名称中的数字表示该滤光片的截止波长（被阻挡光和透射光之间过渡的中点）。带通滤光片透射一个波长带，并阻挡较短和较长的波长。滤光片名称中的数字表示透射带的中心波长和半高宽。通常，在扫描单一荧光染料时，仅需内置的515纳米长波通滤光片。可添加一个可互换滤光片，以减少广谱背景信号的贡献，并衰减强荧光染料信号。
