Romeo D, Zabucchi G, Berton G, Schneider C
J Membr Biol. 1978 Dec 29;44(3-4):321-30. doi: 10.1007/BF01944227.
Polymorphonuclear leucocytes (PMNL) undergo a marked activation of their oxidative metabolism upon interaction with surface-reactive soluble stimuli as well as with phagocytosable objects. To get some insight into the mechanism of this stimulation, we have compared the stimulatory activity of the tetravalent lectin concanavalin A (Con A) with that of the divalent derivative succinyl-Con A (S-Con A). Both lectins bind to the PMNL surface to the same extent. S-Con A, however, is much less efficient in stimulating the PMNL metabolism. When S-Con A-treated PMNL are further reacted with antiserum to Con A, a potentiation of the metabolic stimulation is observed. Normal serum or addition to PMNL of antiserum to Con A in the absence of lectin has no effect. Furthermore, if S-Con A is displaced from its receptors on the cell membrane with alpha-methyl mannopyranoside, the addition of antiserum fails to cause a respiratory stimulation. These results suggest that the initial triggering of the metabolic stimulation of PMNL is in part accomplished through cross-linkage of membrane constituents.
多形核白细胞（PMNL）在与表面反应性可溶性刺激物以及可吞噬物体相互作用时，其氧化代谢会发生显著激活。为了深入了解这种刺激的机制，我们比较了四价凝集素伴刀豆球蛋白A（Con A）与二价衍生物琥珀酰-Con A（S-Con A）的刺激活性。两种凝集素与PMNL表面的结合程度相同。然而，S-Con A在刺激PMNL代谢方面的效率要低得多。当用抗Con A血清进一步处理经S-Con A处理的PMNL时，可观察到代谢刺激的增强。正常血清或在无凝集素的情况下向PMNL中添加抗Con A血清均无作用。此外，如果用α-甲基甘露吡喃糖苷将S-Con A从细胞膜上的受体上置换下来，添加抗血清就不会引起呼吸刺激。这些结果表明，PMNL代谢刺激的初始触发部分是通过膜成分的交联来完成的。
