Freze K V, Danilkovich A V, Kharitonenkov A I, Shevalier A F, Surin A M, Khodorova A B, Astashkin E I, Bulargina T V
Department of Cell Physiology and Immunology, Biological Faculty, Moscow State University, Russia.
Biochem Mol Biol Int. 1994 Aug;33(5):969-79.
A synthetic peptide (designated 2438) corresponding to the human interferon alpha-2 amino acid sequence 124-138 inhibits proliferation of T-lymphocytes in vitro. Time-course experiments suggest that peptide 2438 affects early stages of lymphocyte activation. Molecular mechanisms of peptide 2438 action were studied. By western-blotting with monoclonal antibodies against phosphotyrosine peptide 2438 was shown to decrease the phosphotyrosine content of an endogenous protein substrate (M.M. = 36 kDa) in human lymphocytes activated with concanavalin A (ConA). Similar effect on tyrosine-specific phosphorylation in mitogen-stimulated lymphocytes was observed with the native interferon or Cyclosporine A (CsA). Calcium fluxes induced by ConA in human lymphocytes were measured using a fluorescent calcium chelator Fura-2. In contrast to CsA, peptide 2438 did not affect the ConA-induced calcium influx in lymphocytes.
一种与人类α-2干扰素氨基酸序列124 - 138相对应的合成肽（命名为2438）可在体外抑制T淋巴细胞的增殖。时间进程实验表明，肽2438影响淋巴细胞激活的早期阶段。对肽2438的作用分子机制进行了研究。通过用抗磷酸酪氨酸单克隆抗体进行蛋白质免疫印迹分析，结果显示肽2438可降低经刀豆蛋白A（ConA）激活的人淋巴细胞中一种内源性蛋白质底物（分子量 = 36 kDa）的磷酸酪氨酸含量。在用天然干扰素或环孢素A（CsA）处理有丝分裂原刺激的淋巴细胞时，观察到了对酪氨酸特异性磷酸化的类似作用。使用荧光钙螯合剂Fura-2测量ConA诱导的人淋巴细胞中的钙通量。与CsA不同，肽2438不影响ConA诱导的淋巴细胞钙内流。
