Yoshida K, Okamura T, Toda N
Department of Pharmacology, Shiga University of Medical Sciences, Ohtsu, Japan.
Jpn J Pharmacol. 1994 Aug;65(4):351-9. doi: 10.1254/jjp.65.351.
Nitroxidergic nerves and their functional role were determined in a variety of monkey arteries. Nitric oxide synthase-immunoreactive nerve fibers innervating the monkey arterial wall were histochemically determined by the use of nitric oxide synthase antiserum. Thin nitric oxide synthase-immunoreactive fibers were consistently found in the outer media of monkey cerebral, mesenteric and temporal arteries, in addition to many thicker fibers and nerve bundles in the adventitia. In the monkey pterygopalatine ganglion, the immunoreactivity was clearly seen in nerve cells, bundles and fibers. Helical strips of monkey arteries were exposed to the bathing media for tension recordings and were stimulated by electrical square pulses. In helical strips of the cerebral artery denuded of the endothelium, transmural electrical stimulation produced relaxations that were abolished by tetrodotoxin or NG-nitro-L-arginine, a nitric oxide synthase inhibitor. In the monkey mesenteric and temporal arterial strips treated with alpha-adrenoceptor antagonists, the relaxation caused by electrical stimulation was also abolished by the nitric oxide synthase inhibitor, and it was restored by L-arginine. Nitroxidergic perivascular nerves, histologically demonstrated, appear to play an important role in dilating the monkey cerebral artery and in counteracting a vasoconstriction associated with noradrenergic nerve activation in the mesenteric and temporal arteries.
在多种猴类动脉中确定了含氮氧化物的神经及其功能作用。通过使用一氧化氮合酶抗血清，对支配猴动脉壁的一氧化氮合酶免疫反应性神经纤维进行了组织化学测定。除了外膜中有许多较粗的纤维和神经束外，在猴大脑、肠系膜和颞动脉的中膜外层始终能发现细的一氧化氮合酶免疫反应性纤维。在猴翼腭神经节中，神经细胞、神经束和神经纤维中均可见明显的免疫反应性。将猴动脉的螺旋条暴露于浴液中进行张力记录，并用方形电脉冲进行刺激。在去除内皮的大脑动脉螺旋条中，经皮电刺激可产生舒张反应，该反应可被河豚毒素或一氧化氮合酶抑制剂NG-硝基-L-精氨酸消除。在用α-肾上腺素能受体拮抗剂处理的猴肠系膜和颞动脉条中，电刺激引起的舒张反应也可被一氧化氮合酶抑制剂消除，并可被L-精氨酸恢复。组织学证实，含氮氧化物的血管周围神经在扩张猴大脑动脉以及对抗肠系膜和颞动脉中与去甲肾上腺素能神经激活相关的血管收缩方面似乎起着重要作用。
