南非黑人儿童中OKT4表位缺陷的流式细胞术分析
Flow cytometry analysis of OKT4 epitope deficiency in South African black children.
作者信息
Hughes E J, Goddard E A, Bouic P, Beatty D W
机构信息
Department of Paediatrics and Child Health, Institute of Child Health, Rondebosch, South Africa.
出版信息
Clin Exp Immunol. 1994 Dec;98(3):526-31. doi: 10.1111/j.1365-2249.1994.tb05523.x.
A case of OKT4 epitope deficiency referred for investigation with suspected immunodeficiency is described. Flow cytometry analysis of OKT4 epitope deficiency in a study group of healthy black children showed different manifestations of the lack of OKT4 epitope; a complete lack of OKT4+CD4+ peripheral blood lymphocytes (PBL) with normal numbers of OKT4A+ and Leu3a-CD4+ PBL, decreased percentage OKT4+CD4+ compared with OKT4A+ and Leu-3a+CD4+ PBL, decreased fluorescent staining intensity with OKT4 and a biphasic OKT4 staining pattern associated with a reduced OKT4/Leu-3a ratio. The percentage and fluorescent intensity of OKT4+CD4+ PBL in the study group were significantly lower (P < 0.0001) than Leu-3a+CD4+ and OKT4A+CD4+ PBL. There is thus considerable risk of under-estimating the number of CD4+ cells in black South Africans if the OKT4 MoAb is used.
本文描述了一例因疑似免疫缺陷而转诊进行检查的OKT4表位缺陷病例。对一组健康黑人儿童进行的OKT4表位缺陷的流式细胞术分析显示，OKT4表位缺乏有不同表现：OKT4+CD4+外周血淋巴细胞（PBL）完全缺乏，而OKT4A+和Leu3a-CD4+ PBL数量正常，与OKT4A+和Leu-3a+CD4+ PBL相比，OKT4+CD4+百分比降低，OKT4荧光染色强度降低，以及与降低的OKT4/Leu-3a比值相关的双相OKT4染色模式。研究组中OKT4+CD4+ PBL的百分比和荧光强度显著低于Leu-3a+CD4+和OKT4A+CD4+ PBL（P < 0.0001）。因此，如果使用OKT4单克隆抗体，在南非黑人中存在低估CD4+细胞数量的相当大风险。
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