Dimri R, Nissimov L, Keisari Y
Department of Human Microbiology, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Lymphokine Cytokine Res. 1994 Aug;13(4):239-45.
Granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-3, which are involved in the maturation of cell precursors in the bone marrow into granulocytes and macrophages, were found also in chronic inflammatory sites, and their production might be enhanced by inflammatory stimulants. These findings led us to examine the effect of human recombinant GM-CSF (hrGM-CSF) and hrIL-3 on the maturation of human peripheral blood monocytes in long-term tissue cultures and on the expression of functional membrane bound molecules. Adherent human peripheral blood monocytes cultured for 2 weeks in the presence of GM-CSF or IL-3 were examined for viability and adherence, expression of membranal HLA-DR, CD-14, and IL-1 alpha, and LPS triggered TNF-alpha production. GM-CSF and IL-3 treatment increased the viability of adherent cells after 2 weeks in culture, and elevated the expression of membranal HLA-DR, CD-14 (LPS receptor), and IL-1 alpha. Such treated macrophage cultures also showed elevated production of TNF-alpha. The results indicate that GM-CSF and IL-3 facilitate the long-term maturation of monocytes into macrophages, augment their capacity to bind LPS, and elevate the release of cytokines involved in inflammatory and granulomatous reactions.
粒细胞巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-3（IL-3）参与骨髓中细胞前体向粒细胞和巨噬细胞的成熟过程，在慢性炎症部位也有发现，且炎症刺激物可能会增强它们的产生。这些发现促使我们研究重组人GM-CSF（hrGM-CSF）和hrIL-3在长期组织培养中对人外周血单核细胞成熟以及功能膜结合分子表达的影响。对在GM-CSF或IL-3存在下培养2周的贴壁人外周血单核细胞进行活力、贴壁情况、膜表面HLA-DR、CD-14和IL-1α的表达以及脂多糖触发的肿瘤坏死因子-α（TNF-α）产生的检测。GM-CSF和IL-3处理可提高培养2周后贴壁细胞的活力，并增加膜表面HLA-DR、CD-14（脂多糖受体）和IL-1α的表达。经此类处理的巨噬细胞培养物也显示出TNF-α产生增加。结果表明，GM-CSF和IL-3促进单核细胞长期成熟为巨噬细胞，增强其结合脂多糖的能力，并提高参与炎症和肉芽肿反应的细胞因子的释放。
