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Cloning of the hsp70 (dnaK) genes from Rhizobium meliloti and Pseudomonas cepacia: phylogenetic analyses of mitochondrial origin based on a highly conserved protein sequence.来自苜蓿根瘤菌和洋葱伯克霍尔德菌的hsp70（dnaK）基因的克隆：基于高度保守蛋白质序列的线粒体起源系统发育分析。
J Bacteriol. 1994 Dec;176(24):7748-53. doi: 10.1128/jb.176.24.7748-7753.1994.
2
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The sequences of heat shock protein 40 (DnaJ) homologs provide evidence for a close evolutionary relationship between the Deinococcus-thermus group and cyanobacteria.热休克蛋白40（DnaJ）同源物的序列为嗜热栖热菌属与蓝细菌之间密切的进化关系提供了证据。
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Sequencing of heat shock protein 70 (DnaK) homologs from Deinococcus proteolyticus and Thermomicrobium roseum and their integration in a protein-based phylogeny of prokaryotes.嗜热栖热菌和玫瑰嗜热栖微菌热休克蛋白70（DnaK）同源物的测序及其在基于蛋白质的原核生物系统发育中的整合。
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Presence of a mitochondrial-type 70-kDa heat shock protein in Trichomonas vaginalis suggests a very early mitochondrial endosymbiosis in eukaryotes.阴道毛滴虫中存在线粒体型70 kDa热休克蛋白，这表明真核生物中存在非常早期的线粒体内共生现象。
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The dnaKJ operon of Agrobacterium tumefaciens: transcriptional analysis and evidence for a new heat shock promoter.根癌土壤杆菌的dnaKJ操纵子：转录分析及新热休克启动子的证据
J Bacteriol. 1995 Oct;177(20):5952-8. doi: 10.1128/jb.177.20.5952-5958.1995.

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On the evolutionary origin of the plant mitochondrion and its genome.植物线粒体及其基因组的进化起源。
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Cloning of Giardia lamblia heat shock protein HSP70 homologs: implications regarding origin of eukaryotic cells and of endoplasmic reticulum.蓝氏贾第鞭毛虫热休克蛋白HSP70同源物的克隆：对真核细胞起源和内质网起源的启示
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2895-9. doi: 10.1073/pnas.91.8.2895.
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来自苜蓿根瘤菌和洋葱伯克霍尔德菌的hsp70（dnaK）基因的克隆：基于高度保守蛋白质序列的线粒体起源系统发育分析。

Cloning of the hsp70 (dnaK) genes from Rhizobium meliloti and Pseudomonas cepacia: phylogenetic analyses of mitochondrial origin based on a highly conserved protein sequence.

作者信息

Falah M, Gupta R S

机构信息

Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.

出版信息

J Bacteriol. 1994 Dec;176(24):7748-53. doi: 10.1128/jb.176.24.7748-7753.1994.

DOI:10.1128/jb.176.24.7748-7753.1994
PMID:7528198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC197237/
Abstract

The genes for hsp70 (or dnaK) have been cloned and sequenced from Rhizobium meliloti and Pseudomonas cepacia, two bacterial species belonging to the alpha- and beta-subdivisions of gram-negative proteobacteria, respectively. On the basis of global alignment of HSP70 proteins, several sequence signatures have been identified that are distinctive of mitochondrial homologs and gram-negative proteobacteria on the one hand and the chloroplasts and cyanobacteria on the other. Detailed phylogenetic analyses of HSP70 sequences from various eubacteria and eukaryotic organellar and cytosolic homologs support the inference regarding the origin of mitochondria from a member of the alpha-proteobacteria and of chloroplasts from cyanobacteria. The analysis presented here also suggests a monophyletic origin of the mitochondrial homologs.

摘要

热休克蛋白70（或dnaK）的基因已从苜蓿根瘤菌和洋葱假单胞菌中克隆并测序，这两种细菌分别属于革兰氏阴性变形菌的α-和β-亚群。基于HSP70蛋白的全局比对，已鉴定出几个序列特征，一方面是线粒体同源物和革兰氏阴性变形菌所特有的，另一方面是叶绿体和蓝细菌所特有的。对来自各种真细菌、真核细胞器和胞质同源物的HSP70序列进行的详细系统发育分析支持了关于线粒体起源于α-变形菌的一个成员以及叶绿体起源于蓝细菌的推断。此处给出的分析还表明线粒体同源物有一个单系起源。