Cridland J C, Booth V, Ashley F P, Curtis M A, Wilson R F, Shepherd P
Department of Immunology, United Medical School, Guy's Hospital, London, England.
J Periodontal Res. 1994 Sep;29(5):339-47. doi: 10.1111/j.1600-0765.1994.tb01232.x.
A panel of 15 monoclonal antibodies (MAbs) was raised to Porphyromonas gingivalis and used to characterise the antigens which they recognise by ELISA, immunofluorescence (IF), Western blotting and patient serum inhibition studies. All the MAbs were specific for P. gingivalis and did not recognise 24 other species. Eight MAbs gave bright ring-like staining patterns against the majority of serotypes of P. gingivalis tested by IF. The antibodies could be grouped into 5 different antigen recognition profiles on Western blotting, and ELISA indicated that 8 of them bound to a capsular extract. Five antibodies bound to antigenic determinants recognised by sera from patients with periodonitis and 4 of these (1A1, 2B/H9, 3B1, 7D5) bound to a P. gingivalis 47kDa protease preparation on Western blotting. These antibodies are potentially useful for the purification and characterisation of biologically active components of P. gingivalis that are recognised by sera from patients with periodontitis.
制备了一组针对牙龈卟啉单胞菌的15种单克隆抗体（MAb），并用于通过酶联免疫吸附测定（ELISA）、免疫荧光（IF）、蛋白质印迹法和患者血清抑制研究来表征它们所识别的抗原。所有单克隆抗体对牙龈卟啉单胞菌具有特异性，不识别其他24种菌。通过免疫荧光法检测，8种单克隆抗体对大多数牙龈卟啉单胞菌血清型呈现明亮的环状染色模式。在蛋白质印迹法中，这些抗体可分为5种不同的抗原识别谱，酶联免疫吸附测定表明其中8种与荚膜提取物结合。5种抗体与牙周炎患者血清识别的抗原决定簇结合，其中4种（1A1、2B/H9、3B1、7D5）在蛋白质印迹法中与牙龈卟啉单胞菌47kDa蛋白酶制剂结合。这些抗体可能有助于纯化和表征牙周炎患者血清所识别的牙龈卟啉单胞菌生物活性成分。
