Bileviciute I, Lundeberg T, Ekblom A, Theodorsson E
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Regul Pept. 1994 Aug 31;53(1):71-6. doi: 10.1016/0167-0115(94)90160-0.
Substance P (SP)-, neurokinin A (NKA)-, calcitonin gene-related peptide (CGRP)- and neuropeptide Y (NPY)-like immunoreactivity (-LI) was studied in rats' cerebrospinal fluid (CSF), plasma and synovial fluid (SF) from both knee joints at 2 and 24 h following an intraperitoneal administration of 0.05 ml human recombinant interleukin-1 alpha (i.p. hrIL-1 alpha) or saline. Increased or decreased levels of SP-, NKA and CGRP-LI were detected in CSF and plasma, whereas NPY-LI was unaffected. In SF only CGRP-LI increased bilaterally. There was a correlation in CGRP-LI content between plasma and CSF following i.p. hrIL-1 alpha but not between plasma and SF or CSF and SF. It can be concluded that (1) i.p. hrIL-1 alpha activates somatosensory afferents thereby increasing SP- and CGRP-LI content in CSF and plasma and NKA-LI in CSF; 2) i.p. hrIL-1 alpha induces a bilateral increase of CGRP-LI in SF which is not mediated through systemic circulation and is possibly a part of the general host defensive reaction.
在腹腔注射0.05 ml人重组白细胞介素-1α（腹腔注射hrIL-1α）或生理盐水后2小时和24小时，对大鼠的脑脊液（CSF）、血浆以及双侧膝关节的滑液（SF）中的P物质（SP）、神经激肽A（NKA）、降钙素基因相关肽（CGRP）和神经肽Y（NPY）样免疫反应性（-LI）进行了研究。在脑脊液和血浆中检测到SP-、NKA和CGRP-LI水平升高或降低，而NPY-LI未受影响。在滑液中，仅双侧CGRP-LI增加。腹腔注射hrIL-1α后，血浆和脑脊液中的CGRP-LI含量存在相关性，但血浆与滑液或脑脊液与滑液之间不存在相关性。可以得出结论：（1）腹腔注射hrIL-1α激活躯体感觉传入神经，从而增加脑脊液和血浆中SP-和CGRP-LI的含量以及脑脊液中NKA-LI的含量；（2）腹腔注射hrIL-1α诱导滑液中CGRP-LI的双侧增加，这不是通过体循环介导的，可能是全身宿主防御反应的一部分。
