Benelli R, Repetto L, Carlone S, Parravicini C, Albini A
Istituto Nazionale per la Ricerca sul Cancro, IST, Genova, Italy.
Res Virol. 1994 May-Aug;145(3-4):251-9. doi: 10.1016/s0923-2516(07)80030-6.
We have established and characterized two new Kaposi's sarcoma (KS) cell lines derived from skin biopsies: AIDS-KSISTIV (from an AIDS-associated KS) and KSISTVIII (from a sporadic KS). AIDS-KSISTIV and KSISTVIII are composed mostly of spindle-shaped cells. They show similar patterns of immunohistochemical staining and are positive for smooth muscle (smooth muscle alpha-actin) and fibroblastoid (TE7) markers. Neither of these lines express the endothelial marker von Willebrand factor VIII. These immunohistochemical patterns are similar to numerous other KS lines that we and others have established. When seeded on a reconstituted basement membrane ("Matrigel"), AIDS-KSISTIV and KSISTVIII cells form branching colonies and invade into the Matrigel, as do other KS cultures that we have previously examined. This behaviour on Matrigel is similar to that of malignant sarcoma cells of different origin. The expression of vimentin and the morphology of the invasive colonies on Matrigel suggest that KS-derived cells are poorly differentiated mesenchymal cells. KS lesions are characterized by a conspicuous neovascularization, which appears to be derived from host cell recruitment. We tested the capability of the KS-cell supernatants to induce an angiogenic response in vitro. The new lines are able to stimulate human endothelial cell chemotaxis and invasion through Matrigel-coated filters. No differences in angiogenic potential in vitro were observed between the AIDS and the non-AIDS case, as we previously noted for other established cultures. Our new lines have the properties of true KS cells and confirm that KS spindle cells from HIV-positive or -negative patients have identical phenotypic and behavioural characteristics in vitro.
我们已建立并鉴定了两种源自皮肤活检的新型卡波西肉瘤（KS）细胞系：AIDS-KSISTIV（源自与艾滋病相关的KS）和KSISTVIII（源自散发型KS）。AIDS-KSISTIV和KSISTVIII主要由梭形细胞组成。它们显示出相似的免疫组织化学染色模式，对平滑肌（平滑肌α-肌动蛋白）和成纤维细胞样（TE7）标志物呈阳性。这两种细胞系均不表达内皮标志物血管性血友病因子VIII。这些免疫组织化学模式与我们和其他人建立的许多其他KS细胞系相似。当接种在重组基底膜（“基质胶”）上时，AIDS-KSISTIV和KSISTVIII细胞形成分支菌落并侵入基质胶，我们之前检测过的其他KS培养物也是如此。在基质胶上的这种行为与不同来源的恶性肉瘤细胞相似。波形蛋白的表达以及基质胶上侵袭菌落的形态表明，源自KS的细胞是低分化的间充质细胞。KS病变的特征是明显的新生血管形成，这似乎源于宿主细胞的募集。我们测试了KS细胞上清液在体外诱导血管生成反应的能力。新的细胞系能够刺激人内皮细胞趋化并通过基质胶包被的滤膜侵袭。在艾滋病和非艾滋病病例之间未观察到体外血管生成潜力的差异，正如我们之前对其他已建立的培养物所指出的那样。我们的新细胞系具有真正KS细胞的特性，并证实来自HIV阳性或阴性患者的KS梭形细胞在体外具有相同的表型和行为特征。
