绵羊粒细胞集落刺激因子cDNA的克隆与测序

Cloning and sequencing of an ovine granulocyte colony-stimulating factor cDNA.

作者信息

O'Brien P M, Seow H F, Rothel J S, Wood P R

机构信息

CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.

出版信息

DNA Seq. 1994;4(5):339-42. doi: 10.3109/10425179409020862.

DOI:10.3109/10425179409020862

PMID:7528579

Abstract

A cDNA encoding an ovine (ov) granulocyte colony-stimulating factor (G-CSF) was cloned by polymerase chain reaction, using primers based on the 5' and 3' nucleotide (nt) sequence of the bovine (bov) G-CSF cDNA. RNA was isolated from IFN-gamma and LPS-stimulated alveolar macrophages. The isolated ovG-CSF cDNA is 522 bp in length and does not include coding sequence for a secretory signal peptide. The nt sequence is 95% identical to the bovG-CSF nt sequence, and includes conservation of 5 cysteine residues. The deduced amino acid (aa) sequence of the mature ovG-CSF protein shares 96%, 82% and 68% homology with the bov, human and murine mature G-CSF proteins, respectively. The estimated molecular weight of the 174 aa G-CSF protein is 18.8 kilodaltons.

摘要

利用基于牛（bov）粒细胞集落刺激因子（G-CSF）cDNA的5'和3'核苷酸（nt）序列设计的引物，通过聚合酶链反应克隆了编码绵羊（ov）G-CSF的cDNA。从干扰素-γ和脂多糖刺激的肺泡巨噬细胞中分离RNA。分离出的ovG-CSF cDNA长度为522 bp，不包括分泌信号肽的编码序列。该nt序列与bovG-CSF nt序列有95%的同一性，且包含5个半胱氨酸残基的保守序列。成熟ovG-CSF蛋白的推导氨基酸（aa）序列与牛、人和鼠成熟G-CSF蛋白的同源性分别为96%、82%和68%。174个氨基酸的G-CSF蛋白的估计分子量为18.8千道尔顿。