Tang J S, Li C Y, Wang B M
Department of Hematology, First Affiliated Hospital, Guangzhou Medical College.
Zhonghua Zhong Liu Za Zhi. 1994 Jul;16(4):259-62.
Using serum-free culture system and liquid/semi-solid dual culture technique, we examined the effect of rhSCF on self-renewal of the stem/progenitor cells of human acute myelogenous leukemia (AML-CFU). The results revealed that the rhSCF as a hematopoietic growth factor (HGF) exerted its effect hierarchically earlier than interleukin-3 and granulocyte/macrophage colony-stimulating factor on AML-CFU. rhSCF, whether used alone or in combination with other HGFs, showed potential maintenance of self-renewal of AML-CFU in most AML patients studied. However, considerable discrepancy existed in maintenance among various FAB types of patients or even in patients with the same FAB type. It is suggested that SCF may play an important role in the pathological development of AML.
利用无血清培养系统和液体/半固体双重培养技术，我们检测了重组人干细胞因子（rhSCF）对人急性髓性白血病干/祖细胞（AML-CFU）自我更新的影响。结果显示，作为一种造血生长因子（HGF），rhSCF对AML-CFU发挥作用的时间比白细胞介素-3和粒细胞/巨噬细胞集落刺激因子更早。rhSCF无论单独使用还是与其他HGF联合使用，在大多数研究的AML患者中均显示出维持AML-CFU自我更新的潜力。然而，不同FAB分型的患者之间甚至相同FAB分型的患者之间在维持能力上存在显著差异。提示SCF可能在AML的病理发展中起重要作用。
