Yacyshyn B R, Meddings J B
Department of Medicine, University of Alberta, Edmonton, Canada.
Gastroenterology. 1995 Jan;108(1):132-7. doi: 10.1016/0016-5085(95)90017-9.
BACKGROUND/AIMS: Increased intestinal permeability is observed in Crohn's disease and a subset of first-degree relatives. An alteration in isoform expression of the common leukocyte antigen (CD45) is also found in a significant fraction of patients. Because this alteration may be a measure of antigen exposure, the hypothesis of the study was that this alteration would be observed in both patients and relatives of patients with Crohn's disease and that this would correlate with increased intestinal permeability.
Lactulose and mannitol permeability were defined in healthy controls, patients with Crohn's disease, and their first-degree relatives. Simultaneously, peripheral blood was assayed using flow cytometry for CD45RO expression on CD19+ B cells.
A subset of relatives had significantly increased permeability, as did the majority of patients with Crohn's disease. A small fraction of peripheral B cells from controls expressed the CD45 isoform (< 6%). This fraction was significantly increased for patients with Crohn's disease and their relatives. Relatives with no clinical evidence of Crohn's disease were only found to have increased CD45RO expression if they had increased permeability.
Individuals at risk for developing Crohn's disease include a subset with increased intestinal permeability. These people have an associated phenotypic alteration of circulating B cells that is not observed in controls or relatives with normal intestinal permeability.
背景/目的：在克罗恩病患者及其一部分一级亲属中观察到肠道通透性增加。在相当一部分患者中还发现常见白细胞抗原（CD45）的异构体表达发生改变。由于这种改变可能是抗原暴露的一种衡量指标，本研究的假设是，在克罗恩病患者及其亲属中均会观察到这种改变，且这与肠道通透性增加相关。
测定健康对照者、克罗恩病患者及其一级亲属的乳果糖和甘露醇通透性。同时，采用流式细胞术检测外周血中CD19 + B细胞上CD45RO的表达。
一部分亲属的通透性显著增加，大多数克罗恩病患者也是如此。对照者外周血中一小部分B细胞表达CD45异构体（<6%）。克罗恩病患者及其亲属的这一比例显著增加。只有当通透性增加时，无克罗恩病临床证据的亲属才会出现CD45RO表达增加。
有患克罗恩病风险的个体包括一部分肠道通透性增加的人。这些人循环B细胞存在相关的表型改变，而在肠道通透性正常的对照者或亲属中未观察到这种改变。
