Yakar S, Domene H, Meidan R, Cassorla F, Gilad I, Koch I, Laron Z, Roberts C T, LeRoith D, Eshet R
Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Child and Human Development, National Institutes of Health, Bethesda, Maryland.
Horm Metab Res. 1994 Aug;26(8):363-6. doi: 10.1055/s-2007-1001707.
Growth and development of the spleen involves the growth hormone (GH)/insulin-like growth factor-I (IGF-I) axis. To evaluate the molecular mechanism of these effects we studied the effect of hypophysectomy (Hx) and GH replacement therapy on the expression of IGF-I, the IGF-I receptor and IGF-binding protein-2 (IGFBP-2) in juvenile rats. Hx resulted in a 30% reduction in body weight. GH replacement therapy for seven days partially prevented these effects. IGF-I mRNA levels were reduced 30% by Hx, IGFBP-2 mRNA levels fell 50% whereas IGF-I receptor mRNA levels were unaffected. GH therapy prevented the reduction in IGF-I and IGFBP-2 mRNA levels. These results suggest that the GH effect on splenic growth and development is via local (paracrine) IGF-I expression, in addition to any effect by circulating (endocrine) IGF-I.
脾脏的生长和发育涉及生长激素（GH）/胰岛素样生长因子-I（IGF-I）轴。为了评估这些作用的分子机制，我们研究了垂体切除（Hx）和GH替代疗法对幼年大鼠IGF-I、IGF-I受体和胰岛素样生长因子结合蛋白-2（IGFBP-2）表达的影响。垂体切除导致体重降低30%。为期7天的GH替代疗法部分预防了这些作用。垂体切除使IGF-I mRNA水平降低30%，IGFBP-2 mRNA水平下降50%，而IGF-I受体mRNA水平未受影响。GH疗法预防了IGF-I和IGFBP-2 mRNA水平的降低。这些结果表明，GH对脾脏生长和发育的作用是通过局部（旁分泌）IGF-I表达，此外还有循环（内分泌）IGF-I的任何作用。
