生长激素（GH）刺激幼鼠脾脏中胰岛素样生长因子-I（IGF-I）和胰岛素样生长因子结合蛋白（IGFBP）-2基因的表达。

Growth hormone (GH) stimulates insulin-like growth factor-I (IGF-I) and IGF-binding protein (IGFBP)-2 gene expression in spleens of juvenile rats.

作者信息

Yakar S, Domene H, Meidan R, Cassorla F, Gilad I, Koch I, Laron Z, Roberts C T, LeRoith D, Eshet R

机构信息

Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Child and Human Development, National Institutes of Health, Bethesda, Maryland.

出版信息

Horm Metab Res. 1994 Aug;26(8):363-6. doi: 10.1055/s-2007-1001707.

DOI:10.1055/s-2007-1001707

PMID:7528707

Abstract

Growth and development of the spleen involves the growth hormone (GH)/insulin-like growth factor-I (IGF-I) axis. To evaluate the molecular mechanism of these effects we studied the effect of hypophysectomy (Hx) and GH replacement therapy on the expression of IGF-I, the IGF-I receptor and IGF-binding protein-2 (IGFBP-2) in juvenile rats. Hx resulted in a 30% reduction in body weight. GH replacement therapy for seven days partially prevented these effects. IGF-I mRNA levels were reduced 30% by Hx, IGFBP-2 mRNA levels fell 50% whereas IGF-I receptor mRNA levels were unaffected. GH therapy prevented the reduction in IGF-I and IGFBP-2 mRNA levels. These results suggest that the GH effect on splenic growth and development is via local (paracrine) IGF-I expression, in addition to any effect by circulating (endocrine) IGF-I.

摘要

脾脏的生长和发育涉及生长激素（GH）/胰岛素样生长因子-I（IGF-I）轴。为了评估这些作用的分子机制，我们研究了垂体切除（Hx）和GH替代疗法对幼年大鼠IGF-I、IGF-I受体和胰岛素样生长因子结合蛋白-2（IGFBP-2）表达的影响。垂体切除导致体重降低30%。为期7天的GH替代疗法部分预防了这些作用。垂体切除使IGF-I mRNA水平降低30%，IGFBP-2 mRNA水平下降50%，而IGF-I受体mRNA水平未受影响。GH疗法预防了IGF-I和IGFBP-2 mRNA水平的降低。这些结果表明，GH对脾脏生长和发育的作用是通过局部（旁分泌）IGF-I表达，此外还有循环（内分泌）IGF-I的任何作用。