Reddy M N, Franciosi R A
Department of Pathology, Medical College of Wisconsin, Milwaukee.
Ann Clin Lab Sci. 1994 Sep-Oct;24(5):401-6.
The feasibility of isoelectric focusing (IEF) to determine hemoglobin S (HbS) at a faster turn-around-time and to resolve the HbS and hemoglobin A (HbA) in presence of high concentrations of hemoglobin F (HbF) is evaluated. The IEF procedure is faster, and the results can be obtained in less than 45 minutes. The resulting data are comparable to gel electrophoresis. It is a superior procedure in resolving both HbS and HbA in the presence of high HbF and, therefore, a desirable technique to use for infants and children. Further, IEF is simpler than the gel electrophoresis, relatively inexpensive, easily adaptable for routine use, and suitable for "stat" conditions.
评估了等电聚焦（IEF）在更短周转时间内测定血红蛋白S（HbS）以及在高浓度血红蛋白F（HbF）存在的情况下分辨HbS和血红蛋白A（HbA）的可行性。IEF程序更快，可在不到45分钟内获得结果。所得数据与凝胶电泳相当。在高HbF存在的情况下，它在分辨HbS和HbA方面是一种更优的程序，因此是用于婴幼儿的理想技术。此外，IEF比凝胶电泳更简单，成本相对较低，易于适应常规使用，并且适用于“急诊”情况。
