Obradovich D, Borodin A M, Kopantsev E P, Vagner L L, Volik S V, Ermolaeva O D, Lebedev Iu B, Monastyrskaia G S, Sverdlov E D
Bioorg Khim. 1994 Aug-Sep;20(8-9):919-31.
A new technique based on Alu-PCR amplification of hn-RNA is described for the extraction of human-specific transcribed sequences from a hybrid cell line. Arrayed library of hn-cDNA was constructed and characterized by sequencing about 80 individual clones. A high enrichment by human-specific sequences (about 95%) was demonstrated.
描述了一种基于Alu-PCR扩增hn-RNA的新技术，用于从杂交细胞系中提取人特异性转录序列。构建了hn-cDNA的阵列文库，并通过对约80个单个克隆进行测序来表征。结果表明，人特异性序列的富集度很高（约95%）。
