Tucker R P, Brunso-Bechtold J K, Jenrath D A, Khan N A, Poss P M, Sweatt A J, Xu Y
Department of Neurobiology and Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.
Perspect Dev Neurobiol. 1994;2(1):89-99. doi: 10.1080/0907676x.1994.9961226.
We have used in situ hybridization and reverse transcriptase polymerase chain reaction (PCR) to study the origins of the extracellular matrix glycoprotein tenascin during the development of the central and peripheral nervous systems. Previous studies have shown that neural crest cells migrate along pathways that are lined with tenascin. In situ hybridization, PCR, and western blotting reveal that these cells themselves are a major source of tenascin both in vitro and in the embryo. Thus, tenascin is probably not acting as a guidance molecule but is more likely to be promoting neural crest cell motility in a more general way. Similarly, subpopulations of proliferating and migrating glia make tenascin in the developing central nervous system, as do the radial glia that are used as a substratum for migrating neuronal cell bodies. In the adult, tenascin continues to be expressed in the cerebellum by Golgi epithelial cells. This expression, as well as the expression of tenascin in connective tissue, indicates that this molecule may also be playing a role in regulating differentiation. Finally, the distribution of tenascin transcripts in the developing brain and spinal cord is similar to the distribution of mRNAs encoding receptors for platelet-derived growth factor-AA and basic fibroblast growth factor. In vitro studies indicate that both of these factors are potential regulators of tenascin expression.
我们利用原位杂交和逆转录聚合酶链反应（PCR）来研究细胞外基质糖蛋白腱生蛋白在中枢神经系统和周围神经系统发育过程中的起源。先前的研究表明，神经嵴细胞沿着衬有腱生蛋白的路径迁移。原位杂交、PCR和蛋白质印迹分析显示，这些细胞自身在体外和胚胎中都是腱生蛋白的主要来源。因此，腱生蛋白可能并非作为一种导向分子，而更有可能是以一种更普遍的方式促进神经嵴细胞的运动。同样，在发育中的中枢神经系统中，增殖和迁移的神经胶质细胞亚群会产生腱生蛋白，作为迁移神经元细胞体基质的放射状胶质细胞也会产生腱生蛋白。在成体中，高尔基上皮细胞在小脑中持续表达腱生蛋白。这种表达以及腱生蛋白在结缔组织中的表达表明，该分子可能也在调节分化中发挥作用。最后，腱生蛋白转录本在发育中的脑和脊髓中的分布与编码血小板衍生生长因子-AA和碱性成纤维细胞生长因子受体的mRNA的分布相似。体外研究表明，这两种因子都是腱生蛋白表达的潜在调节因子。
