Brunner T, Mogil R J, LaFace D, Yoo N J, Mahboubi A, Echeverri F, Martin S J, Force W R, Lynch D H, Ware C F
Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, California 92037.
Nature. 1995 Feb 2;373(6513):441-4. doi: 10.1038/373441a0.
A number of murine T-cell hybridomas undergo apoptosis within a few hours of activation by specific antigens, mitogens, antibodies against the T-cell antigen receptor, or a combination of phorbol ester and calcium ionophore. This phenomenon has been extensively studied as a model for clonal deletion in the immune system, in which potentially autoreactive T cells eliminate themselves by apoptosis after activation, either in the thymus or in the periphery. Here we show that the Fas/CD95 receptor, which can transduce a potent apoptotic signal when ligand, is rapidly expressed following activation of T-cell hybridomas, as is its functional, membrane-bound ligand. Interference with the ensuing Fas/Fas-ligand interaction inhibits activation-induced apoptosis. Because T-cell receptor ligation can induce apoptosis in a single T hybridoma cell, we suggest that the Fas/Fas-ligand interaction can induce cell death in a cell-autonomous manner.
许多小鼠T细胞杂交瘤在被特定抗原、有丝分裂原、抗T细胞抗原受体抗体或佛波酯与钙离子载体的组合激活后的几小时内会发生凋亡。这种现象作为免疫系统中克隆缺失的模型已被广泛研究，在该模型中，潜在的自身反应性T细胞在激活后，无论是在胸腺还是在外周，都会通过凋亡消除自身。我们在此表明，Fas/CD95受体在T细胞杂交瘤激活后会迅速表达，其功能性膜结合配体也是如此，当配体存在时，该受体可转导强烈的凋亡信号。干扰随后的Fas/Fas配体相互作用可抑制激活诱导的凋亡。由于T细胞受体连接可在单个T杂交瘤细胞中诱导凋亡，我们认为Fas/Fas配体相互作用可通过细胞自主方式诱导细胞死亡。
