Ferlito S, Di Salvo M M, Attanasio L, Finocchiaro P M, Condorelli P M, Mazzone D
Institute of Medical Clinic I,University of Catarria, Italy.
Panminerva Med. 1994 Jun;36(2):76-9.
The authors carried out an investigation on the "short" and "middle-term" effect of the prostanoid derivate iloprost on some molecular haemostatic markers in a group of peripheral vasculopathic patients with critical limb ischemia. The series consists of 10 patients (6 males, 4 females, age 52 +/- 5) suffering from peripheral obstructive vasculopathy at the III-IV stage by Fontaine. After overnight fasting, each patient was given an intravenous infusion of iloprost lasting six hours at the rate of 2 ng/kg/min and reaching approximately the global dosage of 50 gamma; before and after the infusion a venous blood sample was withdrawn; the experiment was repeated under the same conditions after a four week treatment with the drug administered daily at the same dosage. For each sample the plasma levels of betathromboglobulin (BTG) fibrinopeptide A (FPA), tissue plasminogen activator (tPA), plasminogen activator inhibitor (PAI-I) and D-dimer (D-D) (ELISA, methods, kits Boehringer) were measured. The basal values of BTG, FPA, tPA, PAI-I and D-D were significantly increased compared to those of a control group; after the iloprost infusion (acute effect) significant changes of the BTG, FPA, tPA and PAI-I were not found; D-D only showed a marked reduction (p < 0.05); after the four week treatment with infusion the basal values of BTG, FPA, tPA and PAI-I resulted almost unchanged; D-D only showed a marked reduction (p < 0.05) both as regards the basal value and those after the infusion.(ABSTRACT TRUNCATED AT 250 WORDS)
作者对前列腺素衍生物伊洛前列素对一组患有严重肢体缺血的外周血管病变患者某些分子止血标志物的“短期”和“中期”影响进行了研究。该系列包括10名患者（6名男性，4名女性，年龄52±5岁），患有Fontaine III - IV期外周阻塞性血管病变。在禁食过夜后，每位患者以2 ng/kg/min的速率接受持续6小时的伊洛前列素静脉输注，总量约为50μg；输注前后采集静脉血样；在以相同剂量每日给药进行四周治疗后，在相同条件下重复该实验。对于每个样本，测量血浆中β-血小板球蛋白（BTG）、纤维蛋白肽A（FPA）、组织纤溶酶原激活物（tPA）、纤溶酶原激活物抑制剂（PAI-I）和D-二聚体（D-D）的水平（ELISA法，使用勃林格试剂盒）。与对照组相比，BTG、FPA、tPA、PAI-I和D-D的基础值显著升高；伊洛前列素输注后（急性效应），未发现BTG、FPA、tPA和PAI-I有显著变化；D-D仅显示出明显降低（p<0.05）；在进行四周输注治疗后，BTG、FPA、tPA和PAI-I的基础值几乎未变；D-D无论是与基础值相比还是与输注后的值相比，均仅显示出明显降低（p<0.05）。（摘要截断于250字）
