Miyamoto T, Sasaguri Y, Sugama K, Azakami S, Morimatsu M
Department of Pathology, Kurume University School of Medicine, Japan.
Biochem Mol Biol Int. 1994 Oct;34(3):513-20.
We investigated the expression of the c-kit gene in human aortic endothelial cells. RT-PCR and Northern blot analyses showed that a human aortic endothelial cell line expressed mRNA specific for the c-kit gene. Furthermore, the addition of stem cell factor stimulated the cells to increase the amount of c-kit mRNA transcribed in response to its ligand. In situ hybridization also revealed that endothelial cells in vivo express the c-kit gene.
我们研究了c-kit基因在人主动脉内皮细胞中的表达。逆转录聚合酶链反应（RT-PCR）和Northern印迹分析表明，一种人主动脉内皮细胞系表达c-kit基因特异性的信使核糖核酸（mRNA）。此外，添加干细胞因子可刺激细胞增加对其配体作出反应而转录的c-kit mRNA的量。原位杂交也显示体内的内皮细胞表达c-kit基因。
