Segal G, Ron E Z
Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
J Bacteriol. 1995 Feb;177(3):750-7. doi: 10.1128/jb.177.3.750-757.1995.
The heat shock response of the groESL operon of Agrobacterium tumefaciens was studied at the RNA level. The operon was found to be activated under heat shock conditions and transcribed as a polycistronic mRNA that contains the groES and groEL genes. After activation, the polycistronic mRNA appeared to be cleaved between the groES and groEL genes and formed two monocistronic mRNAs. The groES cleavage product appeared to be unstable and subjected to degradation, while the groEL cleavage product appeared to be stable and became the major mRNA representing the groESL operon after long periods of growth at a high temperature. The polycistronic mRNA containing the groES and groEL genes was the major mRNA representing the groESL operon at a low temperature, and it reappeared when the cells were returned to the lower growth temperature after heat shock induction. These findings indicate that the cleavage event is part of the heat shock regulation of the groESL operon in A. tumefaciens.
在RNA水平上研究了根癌土壤杆菌groESL操纵子的热休克反应。发现该操纵子在热休克条件下被激活，并转录为包含groES和groEL基因的多顺反子mRNA。激活后，多顺反子mRNA似乎在groES和groEL基因之间被切割，形成两个单顺反子mRNA。groES切割产物似乎不稳定并发生降解，而groEL切割产物似乎稳定，并在高温下长时间生长后成为代表groESL操纵子的主要mRNA。含有groES和groEL基因的多顺反子mRNA在低温下是代表groESL操纵子的主要mRNA，并且在热休克诱导后细胞恢复到较低生长温度时重新出现。这些发现表明，切割事件是根癌土壤杆菌中groESL操纵子热休克调节的一部分。
