Tsai S L, Chen P J, Hwang L H, Kao J H, Huang J H, Chang T H, Chen D S
Department of Internal Medicine, National Taiwan University Hospital, Taipei.
J Hepatol. 1994 Sep;21(3):403-11. doi: 10.1016/s0168-8278(05)80320-4.
The immune responses to a hepatitis C virus nonstructural protein (T3Ag) overlapping with the C100-3 antigen were examined in three groups of patients with chronic non-A, non-B hepatitis. Group I included 20 cases positive for both anti-C100-3 and the second-generation anti-HCV test (anti-HCV-II): Group II, five cases with anti-C100-3(-)/anti-HCV-II(+); and Group III, seven cases negative for both tests. HCV RNA was detectable in 20 (100%), 4 (80%) and 0 (0%) patients in each group, respectively. Proliferative responses of peripheral blood mononuclear cells to T3Ag were present in 16 (80%), 3 (60%) and 0 (0%) cases in each group, respectively (p < 0.05). Removal of CD8+ T cells from peripheral blood mononuclear cells resulted in a conversion of unresponsiveness to significant proliferation to T3Ag in the remaining cases in groups I and II, but not in group III. This change paralleled the antigen-induced production of interferon-gamma and interleukin-2, but not interleukin-4. The removal also enhanced the T3Ag-stimulated anti-C100-3 antibody production from cultured peripheral blood mononuclear cells in group II patients. These results indicate that the T3Ag-specific type 1 T helper cells play an important role in regulating anti-C100-3 antibody secretion in hepatitis C patients.
在三组慢性非甲非乙型肝炎患者中，检测了对与C100 - 3抗原重叠的丙型肝炎病毒非结构蛋白（T3Ag）的免疫反应。第一组包括20例抗C100 - 3和第二代抗丙型肝炎病毒检测（抗HCV - II）均为阳性的患者；第二组有5例抗C100 - 3（-）/抗HCV - II（+）的患者；第三组有7例两种检测均为阴性的患者。每组中分别有20例（100%）、4例（80%）和0例（0%）患者可检测到HCV RNA。外周血单个核细胞对T3Ag的增殖反应在每组中分别为16例（80%）、3例（60%）和0例（0%）（p < 0.05）。从外周血单个核细胞中去除CD8 + T细胞后，第一组和第二组中其余病例对T3Ag的无反应性转变为显著增殖，但第三组未出现这种情况。这种变化与抗原诱导的γ干扰素和白细胞介素 - 2的产生平行，但与白细胞介素 - 4无关。去除CD8 + T细胞还增强了第二组患者培养的外周血单个核细胞中T3Ag刺激的抗C100 - 3抗体产生。这些结果表明，T3Ag特异性1型辅助性T细胞在调节丙型肝炎患者抗C100 - 3抗体分泌中起重要作用。
