Ashton I K, Walsh D A, Polak J M, Eisenstein S M
Centre for Spinal Studies, Robert Jones and Agnes Hunt Orthopedic Hospital, Oswestry, UK.
Acta Orthop Scand. 1994 Dec;65(6):635-9. doi: 10.3109/17453679408994620.
The annulus fibrosus of the human intervertebral disc is sparsely innervated, some of the fibers containing substance P. We could demonstrate, by autoradiography, binding sites for substance P localized on the endothelium of small blood vessels in the annulus fibrosus of human intervertebral discs removed during anterior fusion for back pain. In binding inhibition studies, binding of 125I-Bolton Hunter-substance P was inhibited by unlabeled substance P and the related tachykinins neurokinin A and neurokinin B with a rank order of potency substance P > NKA > NKB. Specific binding was reduced > 75 percent by 5'-guanylylimidodiphosphate, indicating G-protein coupling. These features are characteristic of an NK1 receptor through which vascular effects, i.e., vasodilation, plasma extravasation and angiogenesis of substance P, are mediated. The presence of NK1 receptors on blood vessels in the annulus fibrosus may indicate a role for substance P in tissue repair although acute proinflammatory effects may contribute to discogenic pain.
人类椎间盘的纤维环神经支配稀少，部分纤维含有P物质。通过放射自显影，我们能够证明在因背痛而行前路融合术时所切除的人类椎间盘纤维环中，小血管内皮上存在P物质的结合位点。在结合抑制研究中，未标记的P物质以及相关速激肽神经激肽A和神经激肽B可抑制125I - 博尔顿·亨特 - P物质的结合，其效力顺序为P物质 > 神经激肽A > 神经激肽B。5'-鸟苷酰亚胺二磷酸可使特异性结合减少超过75%，表明存在G蛋白偶联。这些特征是NK1受体的特性，通过该受体介导P物质的血管效应，即血管舒张、血浆外渗和血管生成。纤维环血管上存在NK1受体可能表明P物质在组织修复中发挥作用，尽管急性促炎作用可能导致椎间盘源性疼痛。
