Agbedana E O, Oshota J O, Taylor G O
Department of Chemical Pathology, College of Medicine, University of Ibadan, Nigeria.
Afr J Med Med Sci. 1993 Sep;22(3):45-8.
Evaluation of albumin estimation by bromocresol green (BCG) method was carried out in sixty nephrotics and twenty control subjects. In nephrotic syndrome, varies; is directly proportional, to 2-globulin and total cholesterol concentrations were significantly increased, while the mean albumin level was significantly reduced when compared with the corresponding control values. In both control and nephrotics, the determination of serum albumin by the BCG method showed good correlations with values obtained by cellulose acetate electrophoresis using the biuret method to determine the total protein, but the mean value for the nephrotics was higher by an average of 0.4g/100ml. Interference with the BCG reaction by an increased varies; is directly proportional, to 2-globulin level was suggested as a possible explanation for the higher mean albumin level obtained by the BCG method in the nephrotics. Inclusion of 0.8M NaCl in the BCG assay system did not prevent the interference by other proteins. However, this interference could to a large extent, be offset by calibrating with a pool of fresh sera previously determined by electrophoresis.
采用溴甲酚绿（BCG）法对60例肾病患者和20例对照者进行白蛋白测定评估。在肾病综合征中，[此处varies; is directly proportional, to 2-globulin表述有误，可能影响准确理解，暂按原文翻译]；与β2球蛋白直接相关且总胆固醇浓度显著升高，而与相应对照值相比，平均白蛋白水平显著降低。在对照者和肾病患者中，用BCG法测定血清白蛋白与用双缩脲法测定总蛋白的醋酸纤维素电泳所得值显示出良好的相关性，但肾病患者的平均值平均高出0.4g/100ml。β2球蛋白水平升高对BCG反应的干扰被认为是BCG法在肾病患者中获得较高平均白蛋白水平的一个可能解释。在BCG检测系统中加入0.8M NaCl并不能防止其他蛋白质的干扰。然而，通过用先前经电泳测定的新鲜血清池进行校准，这种干扰在很大程度上可以被抵消。
