Tatsuta N, Tezuka T
Department of Dermatology, Kinki University School of Medicine, Osaka, Japan.
J Dermatol Sci. 1994 Oct;8(2):111-8. doi: 10.1016/0923-1811(94)90004-3.
A novel monoclonal antibody to hair keratins (TYHF-1) which recognized a doublet of 43/44-kDa hair keratins in the immunoblot analysis was developed. TYHF-1 strongly reacted with the outer root sheath cells at the region of prekeratinization, the keratogeneous zone and the lower part of the isthmus portion in the indirect immunofluorescent examination. The cells of the inner root sheath and cuticle in the upper bulbar portion were also stained with this antibody. In addition, the tongue and nail matrix were also reacted with TYHF-1. However, TYHF-1 reacted with no epidermal keratins either in the indirect immunofluorescent examination or by immunoblot analysis using plantar epidermis. In addition, the cells of the eccrine and apocrine sweat glands, the epithelia of the buccal mucosa, larynx, tonsil, oesophagus, liver, intestine, gallbladder and urinary bladder demonstrated no positive reaction.
开发了一种针对毛发角蛋白的新型单克隆抗体（TYHF-1），该抗体在免疫印迹分析中识别出43/44-kDa毛发角蛋白的双峰。在间接免疫荧光检查中，TYHF-1与角质化前期区域、角质形成区和峡部下部的外根鞘细胞强烈反应。上球部的内根鞘和角质层细胞也被该抗体染色。此外，舌和甲母质也与TYHF-1发生反应。然而，在间接免疫荧光检查或使用足底表皮的免疫印迹分析中，TYHF-1与表皮角蛋白均无反应。此外，小汗腺和大汗腺的细胞、颊黏膜、喉、扁桃体、食管、肝脏、肠道、胆囊和膀胱的上皮均未显示阳性反应。
