Figarella-Branger D, Vagner-Capodano A M, Bouillot P, Graziani N, Gambarelli D, Devictor B, Zattara-Cannoni H, Bianco N, Grisoli F, Pellissier J F
Department of Pathology and Neuropathology, Hôpital de la Timone, Marseille, France.
Neuropathol Appl Neurobiol. 1994 Oct;20(5):439-47. doi: 10.1111/j.1365-2990.1994.tb00994.x.
PDGFs and their receptors expression were examined in a series of 46 meningiomas by using specific monoclonal antibodies. The immunostaining was quantified by an image analyser and the results correlated with clinical and morphological data (histological type and grade). In addition, since the PDGFB chain is encoded by the c-sis proto-oncogene localized on chromosome 22 and because monosomy 22 has been frequently reported in meningiomas, PDGFs and PDGFRs expression have been correlated with cytogenetic analysis performed in 29 cases. The results demonstrate PDGF A and PDGF B expression in most meningioma specimens and co-expression of these growth factors in numerous cells. PDGF A and B immunoreactivity was related to histological grade. PDGFR beta expression was strong in almost all meningiomas whereas PDGFR alpha was low. PDGFR alpha expression was related to tumour location and grade and PDGFR beta to histological subtype only. The cytogenetic analysis was not related to PDGFB chain expression. Taken together these data further confirm PDGF and PDGFR expression in human meningioma; PDGF may exist as an heterodimer (AB) as well as its receptor. The lack of correlation between cytogenetic analysis and PDGF values, the low level of PDGFB in recurrent meningiomas suggests that it is unlikely that the c-sis proto-oncogene plays an important role in the genesis of meningiomas.
通过使用特异性单克隆抗体，在46例脑膜瘤系列中检测了血小板衍生生长因子（PDGFs）及其受体的表达。通过图像分析仪对免疫染色进行定量，并将结果与临床和形态学数据（组织学类型和分级）相关联。此外，由于PDGFB链由位于22号染色体上的c-sis原癌基因编码，且22号染色体单体在脑膜瘤中经常被报道，因此在29例病例中对PDGFs和PDGFRs的表达与细胞遗传学分析进行了关联。结果显示，大多数脑膜瘤标本中存在PDGF A和PDGF B表达，且这些生长因子在众多细胞中共表达。PDGF A和B的免疫反应性与组织学分级相关。几乎所有脑膜瘤中PDGFRβ表达强烈，而PDGFRα表达较低。PDGFRα表达与肿瘤位置和分级相关，而PDGFRβ仅与组织学亚型相关。细胞遗传学分析与PDGFB链表达无关。综合这些数据进一步证实了人脑膜瘤中PDGF和PDGFR的表达；PDGF可能以异二聚体（AB）及其受体的形式存在。细胞遗传学分析与PDGF值之间缺乏相关性，复发性脑膜瘤中PDGFB水平较低，这表明c-sis原癌基因在脑膜瘤发生中不太可能起重要作用。
